Effects Of Transdermal Reflex Reflex On The Expression Of G1y And GABA In Plasma Of Patients With Post - Stroke

Objectives:to study the effectiveness of acupressure on mitigating the severity of the spasticity of Sprague Dawley rats after stroke; and to study the influence of acupressure on the amount of GABA and Gly in the blood plasma of rats.Method:80rats were randomly divided into four groups. Rats in group one received no treatments and for those in other three groups, MCAO models were established. Rats in group two received no further treatments after the models were successfully developed.3days after the models had been made, rats in group three and group four received the treatment of quadriceps femoris acupressure therapeutic apparatus. The pressure of the apparatus varied from300g to500g, the pressing frequency was one time per five seconds, the pressing depth was10cm. The rats received5days of treatment, once a day and each time lasting for15minutes. Rats in group three and group four were massaged quadriceps femoris tendons and muscle bellies respectively. Rats in group five were received quadriceps femoris muscle stretching treatment for5days. They were lied prone and their legs, except for the hind leg on the affected side, were tied. Their knee joints were made bent to their limits, lasting for10seconds and repeating10times. After all groups finished their treatments, the Modified Ashworth Scale were applied to assess the strain of the quadriceps femoris of rats. ELISA method was adopted to examine the amount change of Gly in the plasma of rats. High performance liquid chromatography was applied to examine the amount change of GABA.Results:(1) muscle tone:the results of assessment according to the Modified Ashworth Scale:the difference between group one and group three, four and five indicates statistic significance (p<0.01). Compared with group two, rats in group three, four and five have much lower muscle tone (p<0.01). The decrease of group three is more obvious than that of group four and five. The results indicate that muscle stretching treatment and massaging quadriceps femoris tendons and muscle bellies can all mitigate the severity of spasticity. Massaging quadriceps femoris tendons is more effective; no noticeable distinguish can be observed between muscle stretching treatment and massaging quadriceps femoris muscle bellies.(2) changes in the amount of Gly and GABA in blood plasma:in one-way ANOVA, according to LSD test, Gly and GABA in the blood plasma of the rats in group two, compared with group one, have largely decreased, indicating the effectiveness of the models. Compared with group two, Gly and GABA in the blood plasma of the rats in group three, four and five have all noticeably increased (p<0.01). The increase in group three is more noticeable than that in group four and five (p<0.01). The results show that muscle stretching treatment and massaging quadriceps femoris tendons and muscle bellies can all increase the amount of Gly and GABA in blood plasma. Massaging quadriceps femoris tendons is more effective; muscle stretching treatment and massaging quadriceps femoris muscle bellies have similar effect.Conclusion:1Homemade rat femoralheadfour muscle by operation treatment instrument can simulate the operation according to the law, to avoid theimpact of human factors on the experimental results.2according to the treatment of tendon muscle spasticity after stroke inrats, can effectivelyimprovethe stroke ratmuscle tension after limbspasticity, reliev e spasm effect.3according to the treatment of tendon muscle spasticity after stroke in rats, can effectively improve the content of Gly in rat plasma, GABA,which may be one of the mechanisms to relieve muscle spasm afterstroke in rats.