| Objective:Through the observation of tumor suppression effect of Baicalin on breast cancer xenograft tumor tissues and the capillary permeability, serum malondialdehyde (MDA), tumor tissue of lysyl oxidase (LOX) and other related indicators of impact, to investigate the effect of Baicalin on the tumor microenvironment, and investigate the possible mechanism of anti breast cancer.Method:By subcutaneous inoculation in nude mice MDA-MB231breast cancer cell lines to establish breast cancer transplantation tumor model, using the random number table method80nude mice were randomly divided into4groups:model group, baicalin, adriamycin group, baicalin+adriamycin group. Baicalin group in vaccination within7day to fill the stomach baicalin aqueous solution (100mg/kg), continuous administration for14days; Adriamycin group in the inoculation intraperitoneal injection of7every three days from the date an adriamycin (5mg/kg),5times total drugs; Model group in vaccination within7day to fill the stomach physiological saline (lOml/kg), for14consecutive days. Baicalin+adriamycin group in vaccination within7day to fill the stomach baicalin injection (100mg/kg),14days in a row, and intraperitoneal injection of adriamycin once every three days5mg/kg, a total of5times. Dosing record during the general life of groups of nude mice, and its dynamic observation, the changes of weight and the growth of the transplanted tumor, measuring the transplanted tumors had product, drawing tumors growth curve. By calculating a tumor-burdened nude mice transplantation tumor volume to detect baicalin in breast cancer tumor suppression function of transplanted tumor; Uv-vis spectrophotometer measured under620nm OD values to reflect the effects of baicalin on blood capillary permeability of the tumor; Using the thiobarbituric acid (TBA) colorimetric assay of Baicalin on nude mice serum malondialdehyde (MDA) effect; immunohistochemical staining in breast cancer lysyl oxidase (LOX) expression.Results:(1) adriamycin group (0.20g), baicalin+adriamycin group (0.13g) tumors (0.51g) in the model group obviously reduce weight, there are significant difference statistically significant (P<0.01), baicalin group (0.29g) tumors had significantly reduce weight compared with model group, the difference was statistically significant (P<0.05); Baicalin+adriamycin group compared with adriamycin group, the difference was statistically significant (P<0.05);(2) the baicalin group (including41.18μg/g) to reduce breast cancer tissue blood capillary permeability, and the model group (including94.56μg/g) compare the difference was statistically significant (P<0.05); Adriamycin group (including119.67μg/g) increased capillary permea-bility in tumor tissues, the difference was statistically significant compared with model group (P<0.05); Baicalin+adriamycin group (including101.28μg/g) increased capillary permeability in tumor tissues, there was no statistically significant difference compared with model group (P>0.05);(3) the baicalin group (2.73nmol/mL) can inhibit the expression of serum MDA, a tumor-burdened nude mice with the model group (4.19nmol/mL) comparative differences significant statistical significance (P<0.01); Adriamycin group (5.54nmol/mL) promote the expression of MDA, compared with model group difference was statistically significant (P<0.05); Baicalin+adriamycin group (4.60nmol/mL) promote the expression of MDA, there was no statistically significant difference compared with model group (P>0.05); (4) the baicalin group (30%) can significantly lower the expression of nude mouse breast cancer tissue lai ammonia acyl oxidase, compared with model group (70%), the difference was statistically significant (P<0.05); Adriamycin group (90%), lai ammonia acyl oxidase expression in tumor tissues increased, compared with model group difference was statistically significant (P<0.05)\Baicalin+adriamycin group (70%) can cut the expression of nude mouse breast cancer tissue lai ammonia acyl oxidase, there was no statistically significant difference compared with model group (P>0.05);Conclusion:Baicalin can inhibit the growth of transplanted tumor of breast cancer, and its mechanism may be that baicalin reduces tumor tissue permeability of capillaries, inhibit expression of nude mice and serum content of malondialdehyde, breast cancer tissues of lysyl oxidase, thereby changing tumor hypoxic microenvironment.
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