| Objective:The mechanism of prevention and cure of cardiovascular diseases in perimenopausal of Tianwangbuxin Dan and its decomposed recipe has deduced by observing its influence on the aorta, serum, hormone, Lipids, and morphology of castration and high-fat fed female rats.Methods:The60SD rats model with artificial menopause ovariectomy preparation method, Vaginal cytology proven successful model. The successful model rats were randomly dividedModel group, Progynova group, Tianwangbuxin Dan whole party groupAll parties reduce the amount of habitat group, All parties to the bellflower group; Separate Sham group, The control group,12in each group. Ovariectomized rats, In addition to the control group fed a normal diet, Each group by feeding high fat diet, Intraperitoneal injection of vitamin D3copy atherosclerosis model, And simultaneously press the group were administered orally. Six weeks later, Heart blood, Measured by radioimmunoassay estrogen levels; Enzymatic determination of plasma lipid. The animals were sacrificed after blood collection, Aorta, The morphological changes were observed by HE staining; Take the uterus, and weighed.Results:(1) Results of the general behavior showed the following factors:Control group, sham group was normal and no significant difference; model group hair dryer, fluffy, very active. Among TCM group showed no significant difference, Comparison with the model group, Chinese medicine groups hair dryer, fluffy, frequent and so was improved; Progynova group and contrast, all parties in rats somewhat quiet.(2) Results of the uterine wet weight showed the following factors:Compared with the control group, the uterine wet weight of sham group was no significant difference (p>0.05); Compared with the model group, the gain of uterine weight of blank was significantly (p<0.05), but the reduce habitat group bellflower contrast to uterine wet weight was no significant difference (p>0.05); Heavy comparison with the model group have each drug group uterine wet weight gain significantly different (p<0.05), Contrast with Chinese groups Progynova uterine wet weight gain (p<0.05).(3) Estrogen effects:In E2levels decrease with the control group comparison model group E2values are highly significant difference (p<0.01), Compare Progynova group and the whole party group E2values have increased a very significant difference (p<0.01) with the model group, Compare Progynova group E2levels and the whole party group increased significantly different (p<0.05), Compare dropped two demolition party groups E2levels were significantly different (p<0.05) and the whole party group, And reduce habitat group contrast to grandiflorum group E2value was not significantly different (p>0.05). In FSH, LH levels have decreased in the model group and control group comparison value E2highly significant difference (p<0.01), And the whole side group comparisons of the two parties split the group FSH, LH value increased (p <0.05), And reduce habitat group comparison group go bellflower FSH, LH value not increased significantly (p>0.05).(4) On four of lipids:Compared with the model group, the drug group TG, TC, LDL-C levels lower, In TG, TC, LDL-C levels, each drug group compared with model group indices were highly significant difference (p<0.01), Demolition party and the whole party group comparing indicators TC, HDL-C (p<0.01) were significantly different. The whole party group on progynova contrast, index TC, HDL-C, LDL-C are more significant difference (p<0.01). Each index level, reducing habitat and to compare asexual bellflower difference (p>0.05).(5) Impact on the morphology of the aorta:Control group, sham group aorta smooth, no plaque forming fatty streaks; Model group showed a lot of fatty streaks plaque, calcification, foam cells; Each drug group showed a small amount of calcification and foam cells, in which the whole party group severity of the lightest, and the two groups were not dismantle parties.Conclusions:(1) Tianwangbuxin Dan whole side can be raised ovariectomized female rats E2, HDL-C levels, Reduce serum LH, FSH, TC, LDL-C, levels, Thereby reducing the aortic wall fatty streak, plaque, foam cells, the formation of calcification. Its intervention perimenopausal cardiovascular disease, the mechanism of development, Sex hormone levels and may adjust, Correct endocrine disorders, improve lipid metabolism, fight against atherosclerosis related.(2) Remove side studies show, All parties group increased E2, HDL-C, Lower TC, LDL-C, Pathological changes in the role of aortic impedance than the two split side groups, Tianwangbuxin Dan The reuse of raw radix rehmanniae, so that the drug Kikyo compatibility provides an experimental basis.
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