Effect Of Yangxian Runzu Pills On TNF - α MRNA And NF - κB MRNA In Conjunctival Epithelial Cells Of Dry Eye

Objective:To investigate the effect of nourishing yin and moisturizing eye Pill on dry eye rat epithelial cells TNF-conjunctival alpha mRNA, NF-kBmRNA.Methods:60healthy male SD rats were randomly divided into normal group, sham operation group, nourishing yin and moisturizing eye pill group, new tear natural group, model group, respectively, expressed by A, B, C, D, E group,12rats in each group (A group, no treatment; group B only open the scrotum, not to remove the testicles, as sham operation group; C, D, E three groups of resected testis and epididymis). Expression of epithelial cells of rat TNF-dry eye conjunctiva alpha mRNA, NF-kBmRNA by in situ hybridization method to observe the administration in March, and in the model is completed in a week and end time for medicine after the completion of a Schirmer test (SIT) and the rupture time of tear film (BUT) examination, diagnosis criteria refer to standard SIT and BUT.Results:the last administration after the completion of TNF-alpha C, D group of dry eye rat conjunctival epithelial cells in mRNA, the positive rate of NF-kBmRNA expression was significantly lower than that in E group (P<0.01), rupture time C, D group Schirmer test, tear film (BUT) were significantly higher than that in E group (P<0.05); C, D group of the drug at the end after the completion of Schirmer test, tear break-up time (BUT) were significantly higher than those after modeling a week (P<0.01).