| Objective: the positive also five soup, from the Qing Dynasty Wang Qingren "YILINGAICUO", composed of 7 Chinese herb Astragalus, angelica tail, radix paeoniae rubra,earthworm, Rhizoma Chuanxiong, safflower, peach kernel, has the effect of Tonifying Qi and activating blood, attending suitable for stroke. ASA has antithrombotic effects in vivo, it could inhibit the platelet release reaction, inhibit platelet aggregation, clinically used for seizure prevention of cardiovascular and cerebrovascular diseases. In this experiment, through the establishment of cerebral ischemia reperfusion injury model in rats. From the point of view of the expression of NOS and NO content, brain cells TXA2/PGI2 balance state of traditional Chinese medicine and Western medicine combined treatment on the inquiry after ischemic stroke on brain protection mechanism.Materials and methods: This study selected 60 clean grade SD rats, weight 180 g + 10 g, male and female. Use were randomly divided into five groups,: sham operation group(15 rats), model group(n = 15), Chinese medicine group(15 rats, Bu Yang Huan Wu Decoction intervention), western medicine group(15 rats aspirin intervention). The adaptability of rats fed for a week. Drug intervention were given traditional Chinese medicine group and Western medicine group,corresponding orally for 1 weeks, 1 times / day. Then the preparation of cerebral ischemia / reperfusion rat model. Immediately after the operation and were taken before, wasassessedmovement of rats usi Materials and methods: This study selected 60 clean grade SD rats, weight 180 g + 10 g, male and female. Use were randomly divided into five groups,: sham operation group(15 rats), model group(n = 15), Chinese medicine group(15 rats, Bu Yang Huan Wu Decoction intervention), western medicine group(15 rats aspirin intervention). The adaptability of rats fed for a week. Drug intervention were given traditional Chinese medicine group and Western medicine group,corresponding orally for 1 weeks, 1 times / day. Then the preparation of cerebral ischemia / reperfusion rat model. Immediately after the operation and were taken before, wasassessedmovement of rats using longa method. Drug intervention after traditional Chinese medicine group,western medicine group to continue to give the corresponding gavage for 3 days, 1 times / day.Late after intragastric administration, take the brain tissue of rats, homogenate supernatant, by enzyme linked immunosorbent assay(ELISA) to detect the NOS, no, PGI2, TXA2 expression changes.Results: 1. Each group of rats with ischemia and reperfusion 2h after longa score, compared with the sham operation group, groups significantly different, with statistical significance P < 0.05; between the other groups on the pairwise comparison, does not have significant difference(P > 0.05). Thatthread method, the successful preparation of the model, comparable between groups.2 groups of rats with ischemia and reperfusion 72 h after longa score, compared with the sham operation group, groups significantly different, with statistical significance(P < 0.05; compared with the model group, compared other differences between each group, with statistical significance(P < 0.05.3. Each rat ischemia and reperfusion 72 h after NOS expression, and the sham operation group, in each group were significantly different, with statistical significance(P < 0.05; compared with the model group, Chinese medicine group, western medicine group, with statistical significance(P < 0.05;4. Each rat ischemia and reperfusion 72 h after no expression level, and the sham operation group, in each group were significantly different, with statistical significance(P < 0.05; compared with the model group, Chinese medicine group, western medicine group, with statistical significance(P < 0.05;5. Each rat ischemia and reperfusion 72 h after TXA2 expression levels, and the sham operation group, in each group were significantly different, with statistical significance(P < 0.05; compared with the model group, Chinese medicine group, western medicine group, with statistical significance(P < 0.05;6. Each rat ischemia and reperfusion 72 h after PGI2 expression levels, and the sham operation group, in each group were significantly different, with statistical significance(P < 0.05; compared with the model group, Chinese medicine group, western medicine group, with statistical significance(P < 0.05;Conclusion: 1. Application thread embolism therapy can successfully replicate rat model of cerebral ischemia reperfusion, after cerebral ischemia and after 2H reperfusion rat model for on limb movement dysfunction.2. Yang also five soup, aspirin and combination therapy to improve cerebral ischemia and reperfusion 72 h rats on limb movement dysfunction.3 positive also five soup can inhibit the expression of NOS was downregulated, inhibition of NOcontent.4 positive also five soup can effectively down regulate the expression of TXA2 content, the up-regulated expression of PGI2 content.
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