Study On The Relationship Between "Yang Wei Injury" And Immune Imbalance In Allergic Purpura

Purpose:This study mainly talks about children who have been diagnosed HSP are healthy children in serum cytokines IL- 6, IL- 17, immunoglobulin Ig A, Ig G, Ig M, C3, complement C4 level of testing to explore HSP spillover "" Yang collaterals injury, blood and immune imbalance relationship.Material and method:1, collect and serum collected 60 cases, record the history of 60 cases and the result of the physical and chemical. Requirements in accordance with the diagnosis into standard(1) at the same time, western medicine allergic purpura with purpura diagnostic criteria of traditional Chinese medicine and western medicine allergic purpura diagnostic criteria;(2) in the ages of 3 and 18 years old between;(3) pre-test not use hormone and immune inhibitor treatment;(4) signed informed consent enters, children and their guardian agreed to take part in this study. At the same time to collect and gather a serum of 30 cases of primary school children, the requirements included in the standard(1) age: the age of 3 to 18.(2) general appearance, nutrition and development in good shape, height, weight, chest circumference, hip circumference, waist circumference, etc all meet the national standards;(3) without congenital diseases and genetic diseases;(4) no allergic diseases and acquired disorders each system;(5). Nearly four weeks without infection;(6). Informed consent. 2, all subjects sterile venous blood collection on an empty stomach in the morning 3 ml, will collect whole blood specimens in serum separation tube placed at room temperature for 2 hours, then 1000 x g centrifugal 20 minutes, take supernatant, puts on the qing- 80 under ℃test. 3, The application of ELISA method to detect cytokine IL- 6, IL- 17 content; Application of immune transmission turbidimetry end point determination method of immune function including immune globulin Ig A, Ig G, Ig M, C3, complement C4. 4, Statistical method: using SPSS17.0 statistical software for data analysis,Results:1, cytokines in children with HSP group serum IL- 6(137.06±109.959 pg/ml), IL- 17(26.75±6.90 pg/ml), humoral immune Ig A(2.18±1.106 g/L), Ig G(10.42±2.110 g/L) level is higher than the healthy group of children with statistical significance(P < 0.05); Children, HSP group and healthy group 2 humoral immunity in serum Ig M, C3, complement C4 level no obvious difference(P > 0.05).Conclusion:1, Children who have been diagnosed with HSP cytokines in serum IL- 6, IL- 17 and humoral immunity of Ig A, Ig G levels higher than that of healthy children, can be concluded that cytokines IL- 6, IL- 17 and humoral immunity of Ig A, Ig G immune globulin process involved in the pathogenesis of HSP. 2, the body’s immune function disorder illustrates purpura "Yang collaterals injured blood spillover", led to the pathogenesis of HSP.