Preparation And Isolation Of Almond Peptides And Their Inhibitory Activity And Activity Of Angiotensin - Converting Enzyme

Almond, kernel of Prunus armeniaca, is known for its high protein/oil content. In the recent years, the healthy food market has been eager for almond products. And the researches on almond have also showed the bioactivity of almond protein in different fields.After the oil expression process, the almond’s scrap was usually used in food producing as a cheap seasoning, or even be used as fodder. In fact, many oil-bearing crops’ scrap also contains a considerable amount of bioactive protein, such as walnuts, peanuts, soybeans, sesames. We need a way to make high value-added products from them.In this study, we concluded an efficient general process of hydrolyzing, extracting and separating almond protein in different levels, in massive amount. At last, we got 7 kinds of almond peptides, and 2 of them showed the high ACE inhibitory rate. Then we decided their amino acid sequences. We named them Pep A & Pep B.While most research on biologically active peptide stays in the in-vitro state, we pushed it up to the in-cell level. After the 3 experiments with HUVEC, Pep A & Pep B. were proved to be able to lower blood pressure in different Mechanisms.Pep A:Met-His-Thr-Asp-Asp(IC50=67.52±0.05μg)Pep B:Gln-His-Thr-Asp-Asp (IC50=43.18±0.07μg)There are four parts in this paper.Part one:reviewReviewed the nutrient value of almond, and current studies on almond, the content of biologically active peptide, all the background knowledge on this study.Part two:the hydrolyze and preparation of almond proteinHydrolyze the almonds. Details on gel chromatography and preparative chromatography.And separate the almond’s peptides with them.Part three:measure the ACE inhibitory rate of almond peptide, and amino acid sequence.Part four:the effect of almond peptide on human umbilical vein endothelial cellsIncludes 3 HUVEC-related experiments, proved the Pep A and Pep B to be active with HUVEC, and they have the possibility to lower the blood pressure.Finally, it summarized the contents of the study.