Effects Of Nicotinamide Mononucleotide Adenylyltransferase 1 On Expression Of TH And Caspase3 Protein In Substantia Nigra Of Chronic Parkinson 's Disease Mouse Model

Aim To investigate the effect of nicotinamide mononucleotide adenylyltransferase(NMNAT1) on the expression of Tyrosine Hydroxylase(TH) and cysteinyl aspartate specific proteinase 3(caspase-3) in the Substantia Nigra(SN) of Chronic MPTP-Induced Parkinson’s disease(PD) Mice.Methods Before a week of inducing PD models, lentiviruses were injected bilaterally into the Substantia Nigra of mice. Mice were divided into 3 groups: vehicle group,MPTP group, NMNAT1 group. The protein levels of TH and caspase-3 were measured by Western blot.Results Compared with MPTP group, TH expression increased(t=7.985,P<0.001)and Caspase-3 expression decreased(t=8.901,P<0.001) in NMNAT1 group.Conclusion The results suggested that NMNAT1 may play an important role in protecting dopaminergic neurons, and this role may be effective to down-regulate the expression of Caspase-3.