Study On The Relationship Between CUGBP1 Overexpression And The Development Of Non - Small Cell Lung Cancer

Objective The multifunctional RNA-binding protein CUGBP1 regulates multiple aspects of nuclear and cytoplasmic messenger RNA(m RNA) processing, including splicing, stabilization, and translation of m RNAs. Previous studies have shown that CUGBP1 is overexpressed in non-smallcell lung cancer(NSCLC) tissues, but the pathological functions of CUGBP1 in tumorigenesis and development are unknown.Methods With immunohistochemical method, we detect CUGBP1 expression in 80 cases of NSCLC tumor tissue and adjacent never received chemotherapy or radiotherapy before. Then, Western Blot, CCK 8 method, flow cytometry and DAPI staining were used to explore the biological functions of CUGBP1 in proliferation, apoptosis, and transformation of epithelial mesenchymal(EMT) in A549 cell lines.Results Using immunohistochemistry, the levels of CUGBP1 expression in NSCLC tissues and adjacent non-cancerous tissues were examined and determined to be associated with differentiation. Short hairpin RNA-induced downregulation of CUGBP1 promoted apoptosis and decreased proliferation in the A549 NSCLC cell line. Moreover, Western blot analysis indicated that the depletion of CUGBP1 increased the protein levels of cyclin D1, BAD, BAX, Jun D, and E-cadherin, while the cyclin B1 level decreased. Knockdown of CUGBP1 decreased β-catenin and vimentin levels and increased Ecadherin expression, suggesting that CUGBP1 may contribute significantly to epithelial to mesenchymal transition(EMT) progression.Conclusion These results demonstrate the importance of CUGBP1 in the biological and pathological functions of NSCLC and indicate its potential as a therapeutic target for NSCLC.