Extraction, Purification, Antioxidant Activity And Liver Activity Of Lignans In

preliminary studies found that another major categories of components-lignanamides have strong free radical scavenging activity in vitro. On the basis of the preliminary experiments, this paper using Box-Behnken Response Surface Methodology (RSM) for the first time to the total lignanamides and cannabisin A as an index, and optimize the the extraction process of the lignanamides of Fructus Cannabis. Determine the extraction process as follows: 70.20% ethanol concentration, feed ratio of 1:7.725, a temperature of 82.72℃. Under these conditions, the total lignanamides content is 1.912%. In large-scale industrial production can be set to 65% ethanol concentration, other conditions remain unchanged, in this condition, the total lignanamides content is 1.910%.For further purification of lignanamides, selected from four kinds of different models of macroporous resin, through the static resin adsorption, adsorption kinetic properties, dynamic properties and dynamic adsorption and desorption rate of performance, to determine D-101 type for separation lignanamides best resin. And contains the sample volume, elution solvents and other parameters of an inspection to determine the lignanamides separation process as follows:sample solution (concentration of 3.978mg/mL) to the PH value of 3.98, the temperature is 55℃, add to the already pre-treatment of D-101 resin (a high adsorption column diameter ratio of 1:7, flow rate 1BV/h) Absorption 0.5h, washed with distilled water to 3BV basic colorless, and then 20% ethanol 3BV elution go disposable eluate with 60% ethanol 3BV elution (flow rate 1BV/h), the collection of eluate, decompression concentrated, dry.This article further on Fructus Cannabis and its lignanamide extract the essence set up a TLC method, and cannabisin A for the content detect indicators, the use of C18 column, acetonitrile-0.1% phosphoric acid aqueous solution (22:78) as mobile phase,255nm as detection wavelength, the two set up a cannabisin A HPLC method. And the study of methodology also established. The results showed that indicators such as the sample repeatability, precision instruments and recovery are in line with the quantitave analysis of requirement can be used as determination of cannabisinA is feasible.In order to explore the antioxidant activity and hepatoprotive of the hemp seed lignanamides, this experiment first study the scavenging activity of crude lignanamides, fine lignanamides purified by macroporous resin and cannabisin A by three diffierent free radical systems such as 1,1-Diphenyl-2-picryl-hydrazyl free radical(DPPH), superoxide anion free radical(O2-) and hydroxide free radical(OH). The results showed that all of them had significant scavenging activity with dose dependent relationship in certain dose range. The scavenging activity of fine lignanamides was strongest.CCl4-induced acute liver injury model study was based on the evaluation of antioxidant activity in vitro, to research the antioxidant activity of hemp seed lignanamides in vivo. The experimental results show that lignanamides compounds with a significant reduction in a aspartate aminotransferase (ALT), alanine aminotransferase (AST), lipid peroxidation product malondialdehyde (MDA) level and to enhance superoxide dismutase (SOD) activity. These biochemical indices prompted hemp seed lignanamides probably through enhancing the body type of the activity of antioxidant enzymes to reduce free radical levels in vivo, effectively reduce the free radical-induced liver cell injury, which has played the role of the liver.