Fundamental Study On Antioxidant Substances

Penthorum chinense Pursh a species from genus Penthorum Gronvexl, Penthorum chinense Pursh,its Branches and leaves are traditionally used for treating.The main active ingredients are flavonoids and organic acids.P. chinense Pursh is especially concerned flavonoids and organic acids have such biological function as lowering transaminase and protecting the liver.In this experiment,the study is about the herbal and extract from P. chinense Pursh,chemical antioxidant activity of free radicals Analog filter system methods is used to activity tracking the parts of antioxidant activity; isolated and identified a number of active ingredients from P. chinense Pursh;set up HPLC analysis method, compared of the main active ingredient content of change,provide a reference of qualifying control methods for medicinal formulation to provide a reference.It will help P. chinense Pursh as extracts,health care products,to promote the use of drugs.The following progress has been made:(1) Chemical antioxidant activity of free radicals analog screening test results show that the extract from P. chinense Pursh has strong scavenging on a variety of free radicals:DPPH free radical, super oxide anion radical, hydroxyl radical, has strong antioxidant activity of capacity, shows dose-effect relationship.The best method, decreased in the order of P. chinense Pursh extracts obtained from ethanol.A polar different part of radical clearance rate was ethyl acetate fraction.(2) Using column chromatography and solvent recrystallization method, isolated and purified 5 compounds from ethyl acetate fraction.On the basis of UV,NMR,MS spectroscopic analysis methods and chemical methods,5 compounds were identified as known compounds,namely, quercetin, kaempferol, pinocembrin-7-O-glucoside,quercitrin,gallic acid.For the first time from this plant found is kaempferol.Comparison vitamin C,quercetin,kaempferol,gallic acid have stronger ability of free radical scavenging activity,lay a foundation for pharmacological tests for the next step.(3) Establish HPLC methods for determination the content of active ingredient in P. chinense Pursh and its extracts.The content of flavonoids and organic acids in P. chinense Pursh which is harvested in different time in Liuyang production base is determinated.The content of flavonoids and organic acids in P. chinense Pursh which are place and growth site comprised.And stability test is done to crude drugs and extracts.