Effect Of Immunization Against Somatostatin Genetic Vaccine On Mice Tissues And Distribution Of Expression Products Of The Vaccine

This study discussed the immunologic mechanism of DNA vaccine and its safety. The somatostatin DNA vaccine without antibiotic resistance gene (pGS/2SS-M4GFP-asd) was injected to the male Kunming mice of 7 weeks old. The technologies of streptavidin-biotin-peroxidase-complex and HE staining techniques were used to detect the somatostatin which was expressed by DNA vaccine. The SS in Kunming mice tissue distribution, descents and ascent dynamics were studied. The results of this study can provide a theory reference to the DNA vaccine safety apply.1 The expression products of somatostatin DNA vaccine in mice was distributed in body tissueIn order to analyze the tissues distribution of SS DNA vaccine expression products, the SS DNA vaccine was injected to the Kunming mice of 7 weeks old. The heart, liver, spleen, lung, kidney, muscle tissue were taken after 24 h,48 h,5 d,7 d,9 d,11d, respectively. The tissues were embedded in paraffin, and then the tissue sections were stained with immunohistochemistry technique. The results indicated that the SS DNA vaccine expressions were mainly distributed in muscle tissue neutrophils, macrophages, fibroblasts, in the spleen macrophages, in the liver cells. However, little was detected in heart, lung, kidney.2 Descents and ascent dynamics of SS DNA vaccine expression products in mice tissuesWe analyzed descents and ascent dynamics of SS DNA vaccine in mice tissues with the result of slice in different experimental time. The results show that the product of SS DNA vaccine was detected. The salmonella were attacked in fibroblasts cytoplasm after 24h of injecting. Immunologic reaction rose peak after 48h and SS positive signal was lost at the 5th day. From 7 d to 11 d in the immune, the expression products of vaccine can be detected in macrophages. 48h to 11 d after immunization, the expression pruducts of vaccine in the spleen of the cytoplasm of macrophages were observed in the microscope. Similarly, after the 48 h immunization, the expression pruducts of vaccine in the cytoplasm of liver cells were observed in the microscope, but only until 7 d.3 Somatostatin DNA vaccine impact mice tissueWe observed and recoreded the whole health of the test mice, and analyzed the results of slice in the different time with hematoxylin-eosin staining in order to analyze live attenuated Salmonella choleraesuis in SS DNA vaccine have toxic effects in mice viable growth and development. The result showed that there was no one dead in the experiment and live attenuated Salmonella choleraesuis caused certain pathological damage on liver, but the first 7 days after immunization in mice liver can damage tissue to repair it and to maintain normal liver function, In this regard, the vaccine is safe. In addition, the vaccine did not have other pathological tissue damage detection.