Study On The Enzyme Linked ImmunoSorbent Assays Of Methaqualone In Animal Edible Tissue

Methaqualone (MTQ) is one derivative of quinazolinone, belonging to nonharbiturate intravenous anesthetic. It's commonly used to cure neurasthenia,insomnia,and also as drugs prior to anesthesia administration in clinical.Dependence or addiction can be formed because of long-term use; therefore it becomes psycho pharmaceutical drugs which are controlled by government. However, due to the simple manufacture craft, methaqualone is mass-produced without government authority, and even has been used to domestic animals industry. Therefore, the residues of methaqualone in animal food must be controlled to protect consumer's health and safety.This article compounded and identified MTQ complete antigens.With this artificial antigen, indirect competitive ELISA (ci-ELISA) was set up for the detection of MTQ residue in edible tissues of animal. Otherwise, the extraction method of MTQ in animal edible tissues was also grading-up.Methaqualone(MTQ) was combined with protein (OVA and BSA)using the N-hydroxysuccinimide activated ester method, which were identified by ultraviolet scan,,infrared spectrogram scan,SDS-PAGE electropheresis and indirect ELISA. In this ci-ELISA, the optimal concentration of coating antigen (MTQ-OVA) was 1.25 ug/ml;the working concentration of polyclonal antibody (PMTQ) was 1:64000 and the optimal working dilution of the HRP-IgG were 1:5000.The linear range for detecting MTQ varied from 0.15ng/ml to 37.97 ng/ml.The linear regression equation in this method was y=-0.2373x+0.5639, r=0.993,with the detection limit was 0.18ng/mL. The average recoveries was 86.09% for detecting MTQ residues in animal edible tissues. This consequence authenticated methaqualone could be reclaimecd well. The coefficients of variation in the different batch were 2.21% to 12.05%,and the coefficient of variations in the same batch was ranged from 5.24% to 10.93%.The results of variations sugested that recovery method had a consummate reproducibility. The cross-reactivity with related analytes of diazepam was all bellow 0.01. The cross-reactivity confirmed that the method had a high specificity and selectivity. It could be used to quickly detect methaqualone in animal edible tissues.