Extraction And Purification Of Potato Glycoalkaloids

According to research trends at home and abroad in recent years, briefly discussed the distribution of glycoalkaloids, chemical structure and types of glycoalkaloids, pharmacological activity of glycoalkaloids, introduced chemical structure of potato glycoalkaloids, summarizes qualitative methods of glycoalkaloids, describes the extraction progress of glycoalkaloids from different extraction solvents and different extraction methods, summarizes the purification methods of glycoalkaloids, compared various methods of content determination, indicated a new approach for comprehensive development and utilization of potato glycoalkaloids.According to references and characteristics of the selected material, considering the extraction of the material economy (convenient to purchase), compared normal potato skins with green potato skin, the content of glycoalkaloids in green potatoskin was significantly high. Therefore, using green potato skin as the extracting materials, In this article, aiming to explore influence factors of potato glycoalkaloids in the aspects of different extraction methods and different extraction solvents, and discusses separation and purification conditions of coarse product using column chromatography.The major contents are described as follows:1.Inspection of chemical composition from raw material and screening of different extraction methodschemical composition of potatoes green skin powder:alkaloids, polysaccharide and nucleoside, saponins, amino acids, peptides and protein, phenols, lactones, coumarin and glycosides, sterols (phytosterols) and terpenoids, volatile oil, triterpenes and glycosides, organic acids, probably contained flavonoids and glycosides, cardiac glycosides, anthraquinones, but oil and tannins is without. Investigated the ultraviolet spectrum analysis method applicability of potato glycoalkaloids, choosing the best extraction method from different extraction methods, extraction efficiency:soxhlet extractiontype of extraction solvent>ultrasonic frequency>extraction time> ratio of solid-liquid, determine the optimal factor level combination A3B4C3D1E3 (extraction time 2h, extraction temperature 50℃, ultrasonic frequency 45Hz, type of extraction solvent ethanol-acid extraction solution, ratio of solid-liquid (m/v) 1:12). In further research of single factors experiment, the optimal conditions of potato glycoalkaloids extraction:ultrasonic frequency 45Hz, extraction time 2.5h, ratio of solid-liquid (m/v) 1:14, extraction temperature 50℃, volume ratio of ethanol-acetic acid (100:30, v/v), Under optimal conditions,, determinated TGA content of potato from different materials. extraction efficiency:tuber of normal potato