Genetic Diversity In Natural Germplasm Of Lentinula Edodes In China By SRAP Marker And IGS2 Analysis

In this study, SRAP markers were utilized to reveal the genetic diversity of 53 strains of Lentinula edodes grown in China, which collected from 9 provinces. A total of 491 DNA bands were detected through 11 primer pairs, of which,95.7% bands were polymorphic. The variations of DNA similarity among the strains reveal that there is great genetic diversity in the natural germplasm of Lentinula edodes in China. According to UPGMA clustering, the 53 strains were classified into 2 groups, and 2 groups also classified into 2 subgroups. Group A1 mainly consisted of strains in the Northwest China and Northeast China; Group A2 mainly consisted of strains in the Northwest China and Central China, while group B comprised the strains of Yunnan Plateau, Hengduanshan mountains and Taiwan. The principal component analysis also classified the strains into 3 groups, support the UPGMA clustering results.Fifty-three wild strains of Lentinula edodes were assessed for DNA polymorphism using IGS2-RFLP technique. According to UPGMA clustering, the 53 strains were classified into 4 groups, Group A1mainly consisted of strains in the Yunnan and Northeast China, group II mainly consisted of strains in the Southwest China, groupâ…¢comprised the strains of Central China, while group IV consisted of strains in Hubei and Shanxi.IGS2 fragments of 9 strains were excised from agarose gel, cloned and sequenced. The results displayed that the SR2 was composed of five type of subreapts, and the subreapt was composed of six type of short repeat elements. The heterogeneous lengths of IGS2 are caused mainly by the number of different types of subrepeats within SR2.The fifty-three strains were cultivated with substitute materials on sawdust, and the fruitbodies were successfully produced byl4 strains. The results of variance analysis showed their agronomic characters, expecially for average fruitbody weight, pileus thickness, pileus diameter and average fruitbody weight per bag, are different. This study could be helpful for hybrid between wild and mass production strains.