A Comparative Proteomic Analysis On New-hatched Silkworm,hemolymph And Ovary Of Bombyx Mori Parthenogenetic Line And Parent Of Amphigenetic Line

Sexual reproduction of animals and plants was wildly distributed in nature and was the mian reproductive way. In nature,a part of animals and plants reproduced by parthenogenesis. Parthenogenesis occurs mainly in the Invertebrata,Lower vertebrates, Spores and of the angiosperms, fish, amphibians and reptiles, etc have also occurred.As a model organism of Lepidoptera and a important economic insect, silkworm reproduced mainly by Sexual reproduction. Partheno- genesis of silkworm is sporadic,the parthenogenetic incidence rate, hatchability of silkworm is very low in nature, therefore there are not wildly application in experiment.Parthenogenetic incidence rate, hatchabilityof silkworm increased after the found of aritificial parthenogenesis(AMP), a lot of works which are about the induce method of aritificial parthenogenesis have done by scholar of diffirent country, and some good induce methods have also been found. The which was established by Astaurov was convenient, safe and effective,and was followed by another scholar so far.There were also some reserchs on parthenogenesis in genetic,celler and molecular level.While a lot of studies about silkworm parthenogenesis have been done, the mechanism of it has not been explained completely. To gain an insight into the causing mechanism silkworm parthenogenesis deeply and selective mechanism, we employed comparative proteomic approach mainlyin new-hatched silkworm, hemolymph and ovary which are from parthenogenetic line wu 14 with high incidence rateand hatchability and parent of amphigenetic line 54A with low incidence rateand hatchability. The main results as follow:1.The new-hatched silkworm proteins of Bombyx mori partheno- genetic line wu 14 and parent of amphigenetic line 54A were compared by two dimensional electrophoresis(2-DE), matrix assisted laser desorption ionization and time of fight mass spectrometry (MALDI-TOF/MS) and bioinformatics analysis.Compared with parent of amphigenetic line 54A, tthere are 19 up-ragulated proteins which included 2 specific express proteins and 25 down-ragulated proteins which included 3 specific delect proteins in parthenogenetic line wu 14. 9 proteins which express highly and are clear and were choosed from above 44 different proteins were analysised by MALDI-TOF/MS, and research the protein data bank by software on the basis of obtained Peptide mass fingerprinting(PMF),and 8 proteins have credible result. This proteins include epidermal cytokeratin 2, muscular protein 20, odorant binding protein, glutathione S-transferase translationally con- trolled tumor protein homolog , cuticular protein RR-1 ,cuticular protein RR-1, beta-actin actin, muscle-type A1 . It could be speculated this 8 proteins might be associated with the regulation of lavare growth of parthenogenetic line.2.To gain an insight into the mechanism of silkworm partheno- genesis, the hemolymph proteins of Bombyx mori parthenogenetic line wu 14 and parent of amphigenetic line 54A were compared by promotemic techniques. 5 different peoteins whose difference expressed continually and 1 protein which express specificly in 54A were found .This 6 different proteins were analysised by MALDI- TOF/MS,and research the protein data bank by software on the basis of obtained Peptide mass fingerprinting(PMF), and 2 proteins have credible result,include hemolymph juvenile hormone binding protein, Chymotrypsin inhibitor-8A. It could be speculated this 2 proteins might be associated with the growth regulation of parthenogenetic line.3.The ovary silkworm proteins in the 5th instar larvae of Bombyx mori parthenogenetic line wu 14 and parent of amphigenetic line 54A were compared by two dimensional electrophoresis(2-DE), matrix assisted laser desorption ionization and time of fight mass spectrometry (MALDI-TOF/MS) and bioinformatics analysis, and find 5 different peoteins whose difference expressed continually and 1 protein which express specificly in 54A. This 6 different proteins wThe ovary silkworm proteins in the 5th instar larvae of Bombyx mori parthenogenetic line wu 14 and parent of amphigenetic line 54A were compared by two dimensional electrophoresis(2-DE), matrix assisted laser desorption ionization and time of fight mass spectrome- try (MALDI-TOF/MS) and bioinformatics analysis, and find 5 diffe- rent peoteins whose difference expressed continually and 1 protein which express specificly in 54A. This 6 different proteins were analysised by MALDI-TOF/MS, and research the protein data bank by software on the basis of obtained Peptide mass fingerprinting(PMF) , and 4 proteins have credible result, include cellular retinoic acid binding protein, actin-depolymerizing factor, heat shock cognate protein 70. It could be speculated this 4 proteins might be associated with the growth regulation of parthenogenetic line.The proteins of new-hatched silkworm, hemolymph and ovary in the 5th instar larvae at various days in two lines were compared by two dimensional electrophoresis(2-DE), and a few different spots were found. The differences of physiologic accormmodation and adapt inthe development of Bombyx mori parthenogenetic line and parent of Amphigenetic line were got across in protein level by the analysis of different spot though matrix assisted laser desorption ionization and time of fight mass spectrometry(MALDI-TOF/MS) and bioinformatics analysis, and gained an insight into the mechanism of silkworm parthenogenesis. In addition, the function of the another different spots in different organs of two lins need research later on .