This paper was conducted to study the shade tolerance, drought tolerance and cold hardiness of Woodwardia japonica, Neolepisorus ovatus and Coniogramme japonica from zhejiang province.1,The study on shade tolerance:3 levels of transmission rate of light was made in this experiment, they were 50%, 25% and 8%, so as to measure the change of leaf traits (leaf length, leaf width), chlorophyll content, photosynthetic physiological characteristics (photosynthetic rate, transpiration rate, stomatal conductance). The results indicated that: with more shading the growth of leaf length and width had different change, and at the levels of 25% and 8% of transmission rate of light, the growth of leaf length and width were obviously higher than that at 50%. And 8% was the most suitable relative light intensity of growth of Neolepisorus ovatus and Coniogramme japonica, while that of Woodwardia japonica was 25%. Besides, with more shading, the chlorophyll content was decreasing, the highest light intensity was at the level of 8% and the lowest was 50%. The daily changes of photosynthetic rate in 3 ferns showed double-peak curves, with obvious phenomenon of photosynthetic "noontime rest". The photosynthetic rate, stomatal conductance and transpiration rate were reducing while shading increasing. According to the changes of indexes, the shading-tolerance ability of different plants under shading stress by Membership function were as follows: Neolepisorus ovatusï¹¥Coniogramme japonicaï¹¥Woodwardia japonica.2,The study on drought tolerance:Three plants were put in Greenhouse and under the drought stress in a natural way, measured physiological and biochemical parameters, chlorophyll fluorescence parameters and leaf water retention ability every five days. The results indicated that: The variation of water retention capacity in leaf of Woodwardia japonica changed significantly, the next was Coniogramme japonica, and the slightest was Neolepisorus ovatus. The comparison groups of relative electric conductivity in the test were persistently increased. While the Chlorophyll content of three plants decreased, Coniogramme japonica changed most. The trend of activity of POD, SOD, the content of MDA and soluble protein rise first and felled finally with drought stress deeply. In detail, the activity of POD and SOD and MDA content of Coniogramme japonica grew rapidly in early time, while the Neolepisorus ovatus and Woodwardia japonica increased slowly. When it comes to the Chlorophyll fluorescence parameters under drought stress, it was affected distinctly. The initial fluorescence(Fo) of three ferns increased rapidly, variable fluorescence (Fv),PSâ…¡potential activity (Fv/Fo), actual photochemical quantum yield (Yield) and rapid light curves of three plants showed downward trend, non-photochemical quenching coefficient (qN) curve showed upward trend. According to the changes of indexes, the drought-tolerance ability of different plants under drought stress condition from strong to weak by membership function were as follows: Woodwardia japonicaï¹¥Neolepisorus ovatusï¹¥Coniogramme japonica.3,The study on cold tolerance:The leaves of plants were put in polyscience programmable temperature controller to conduct cold stress, there were four temperature levels to measure different indexes. The results showed that during the first period of cold stress, the relative conductivity of plants increased slowly, then in a certain range of temperature it rapidly increased, and Coniogramme japonica rised fastest. In the low temperature, the trend of the activity of SOD, POD and the soluble protein increased first and then dropped. By the temperature decreasing, the MDA content in the leaves of three plants raised up. According to the changes of indexes, through membership function, the cold-tolerance decreased as follows: Neolepisorus ovatusï¹¥Woodwardia japonicaï¹¥Coniogramme japonica. |