Rapid Detection Method Of Genetic Modified Soybean And Rice

The transgenic technique brings more opportunities to our life,which makes the improvement of varieties,the inerease of output, the enhancement of quality and resistant. However, more than 200 years industry civilization tells us seience is'a double-blade sword'. While bringing the infinite profit to human beings,science also arouses people's worries. Since the naissance of transgenic technique people were all along thinking and arguing about its food safety,environmental risk,ecology seeurity and social ethics morals. So deteeting teehnique of transgenic Plant became more and more important. Only when we detect transgenic plant exactly can we monitor the commercial transgenic food effeetively. And in the import and export trade, the detection of genetically modified crops is also an important link. The RRS GM soybean and BT63 rice detection of which is genetically modified is the most recent research focus.This paper was established by DNA loop-mediated isothermal amplification method for detection of genetically modified crops, compared with detection methods, like general PCR, fluorescent PCR detection of genetically modified crops, these traditional methods. This method reaches a high sensitivity and high specificity, simple, rapid and the lower cost.First, the sequence of a variety of genetically modified structure analysis and comparison of mutation frequency, fragment analysis, conservative, and then screened using computer simulation to design primers for the end of the energy state analysis, secondary structure analysis, interaction analysis, specific screening. Primers designed to achieve: high coverage, which can amplify all the target fragments; high specificity, that is, to other crops and there will be no amplification of biological gene caused by a possible false positive. Second, the standard method used QPCR compared three different DNA extraction methods, the overall performance and price options that can be applied to the detection kit for practice, for the next step of the experimental operation. Finally, loop-mediated isothermal amplification method cf QPCR standard methods, the specificity and sensitivity experiments, was screened for two endogenous genes, 4 ring detection of exogenous gene mediated isothermal amplification primers. The combination of DNA extraction and detection system of response and detection process, the overall specificity and sensitivity of detection of the EU standard have reached , laid an important foundation for further development of the industry or national standards.