| A series complex cell signal transduction in the plant had been triggered in the resistant responds after inoculated by pathogen. Nitric Oxide (NO) as a novel plant signaling molecule has aroused great concern. In this paper, the IWF (Intercellular washing fluids)-260 of wheat (Triticum aestivum) which was infected with the incompatible leaf rust fungus races 260 (Puccinia triticina) was used as elicitor to stimulate the suspension cells and the changes of NO and the other probable molecular mechanism. Greiss reagent and fluorescent NO probe DAF-2DA were used to detecting of dynamic changes of intracellular NO. Pharmacological experiments were carried out to study the signal interactions between calcium (Ca2+), H2O2 and NO. Cytofluorimetric analysis using the fluorescent probe DAF-2DA scrutinized the change of NO in wheat primary leaves after inoculate leaf rust. The NR activity had also been detected. These results could make a further understanding of the change of NO in hypersensitive reaction (HR).IWF-260 could induce NO outbreak, involved of NOS pathway and NR pathway, and NR pathway is the major route in NO production. The programmed cell death (PCD) of wheat suspension cells which was induced by IWF-260 was distinct declined after treated NO inhibitor, indicted that NO played an important role in the PCD induced by IWF-260. Cytofluorimetric had been detected to followed the change of NO indicted NO was involved in HR. These two results showed an important role which NO played in the resistant responds of wheat.The NO content and the PCD were both declined distinctly with prohibition of influx of extracelluar calcium, which indicated that Ca2+ was closely related to the process of IWF-260 induce NO outbreak and the PCD. So Ca2+ may be the upstream of the NO signal pathway.IWF could induced the outbreak of NO and H2O2 when the wheat responded to elicitor, and NO was involved in the H2O2 production, NO and H2O2 were also closely related.
|
PDF Full Text Request