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Identification Of Host Plant Semiochemicals And The Attraction For Apolygus Lucorum (Meyer-Dür)

Posted on:2012-01-18Degree:MasterType:Thesis
Country:ChinaCandidate:S Q ZhangFull Text:PDF
GTID:2143330332487249Subject:Pesticides
Abstract/Summary:PDF Full Text Request
Since 1990s, area-wide application of transgenic BT cotton in China has effectively controlled the cotton bollworm Helicoverpa armigera with resulting reduction in the amount of broad-spectrum insecticides used in cotton production. This decreased use of insecticides has caused the mirids to be major pest insects on BT cotton in China. The mirids have a wide host range, and can spread to crops, grapes, jujube, etc. In order to determine the Electroantennogram and behavioral responses of Apolygus lucorum to semiochemicals of three host plants. We designed many experiments by Electroantennogram and Y-tube. The main results are summarized as follows:(1) The GC-MS results showed that there are short-medium hydrocarbons, Fatty Acid derivative, Terpenoids, and some Aromatic compounds. But in essential oil of host plants, it is significantly different in different plant.(2) Behavioral assays in the Y-tube olfactometer demonstrated that essential oils of three host plants have a significant role in trapping females. And oil of alfalfa led to strongest preference. Males were not attracted distinctly by any essential oils.(3) Electroantennogram analyses indicated that the females Apolygus lucorum were usually more responsive to (Z)-3-hexenol than males. And the males had the strongest response to (E)-2-hexenal. Behavioral assays in the Y-tube olfactometer demonstrated that females had similar shapes of concentration-response curves to the four plant volatiles, and to Acetophenone 10-5(v/v)led to strongest preference. Males were not attracted distinctly by volatiles.(4) Through Y tube olfactometer 12 high response rates complex formulations which luring rates reached above 60% were filtrated.(5) Trapping experiment indicated that the lures of four volatiles have a significant role in trapping females.
Keywords/Search Tags:Apolygus lucorum, Plant oil, Semiochemicals, Electroantennog
PDF Full Text Request
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