| Rhodiola crenulata (Hook.f.et Thoms.)H. Ohba belonging to the plant family of Crassulaceae, is considered to be a precious medicinal plant by modern medicine, which can adapt to the plateau environment,grown in more than three thousand meters above sea level, Alpine, hypoxia, strong radiation in living conditions make it a particular active substance. This article on the Yunnan, Sichuan, Tibet,11 different populations of natural and artificial cultivation of rhodiola crenulata chemical composition analysis evaluation, in northwest Yunnan Province of rhodiola crenulata in haplocalyx identification, determination of where the content of cadmium and lead, and in northwest of rhodiola crenulata ethanol extracts against TMV activity, for the effective utilization of the plant resources provide a theoretical foundation.In the test batch of rhodiola crenulata, measured total ash content not exceeding 8.0%, Sichuan province for the provided sample content Max 7.243%,Zhongdian donge snowⅢbatch sample content minimum 3.596%. Determination of phenol sulfate crenulata crude polysaccharide, measured the total average crude polysaccharide 2.225%,trekking deqin baimang samples the highest crude polysaccharide 3.071%,Zhongdian HABA samples minimum 1.137%.Using high performance liquid chromatography 11 samples salidroside content, measured the total average salidroside 1.090%,Zhongdian donge snowⅢgranted the highest sample content, Sichuan province 1.807% for drug control sample content minimum 0.519%. The results of the analysis are:11 different populations of rhodiola crenulata. whether natural or artificial culture resource, its ash, crude polysaccharide, sachalinsis no obvious difference.Use of steam distillation from the Northwest crenulata and by gas chromatography-mass spectrometry (GC-MS), on which the analysis of chemical composition. According to General ion flow chromatographic separation figure, a total of 62 peak, identified 45 compounds. The identification of compounds accounted for chromatographic peak area 96.56%. Results show that the essential oils of rhodiola crenulata mainly fatty acid, terpene, alcohol and a small amount of esters, ketones, aldehydes, paraffin,phenols and other ingredients. The comparative analysis is:Northwest crenulata identification of volatile and Han swimming equal people 2005 survey of Tibetan medicine big crenulata consistent results.Inductively Coupled Plasma Atomic emission spectrometry (ICP-AES) detection of Northwest crenulata Cd and Pb in the 2005 edition of the Chinese Pharmacopoeia of heavy metals content standards:Cd≤0.3 mg·kg-1,Pb≤5.0 mg·kg-1.The results of the sample Cd content 0.013 3～0.1624 mg·kg-1,well below the national standard 0.3 mg·kg-1;Pb concentration range 4～2.543 0.265 2 mg·kg-1,significantly lower than the national standard 5.0 mg·kg-1.The measuring sample CD, Pb concentrations are below the pharmacopoeia standards.This article takes a half-leaf, leaf discs method in northwest of rhodiola crenulata ethanol extracts to TMV-infection and added value determination, the experimental results show that the earlier received virus application extracts the preservation and sincere extracts testing followed by virus two method of Northwest crenulata extracts are not inhibitory effect of TMV-infection,and extracts the preservation and viruses, etc volume inoculation of crude extracts concentration reaches 1mg·ml-1 is showing 100 %s of inhibition of bacterial plaque. Northwest crenulata extracts 0.1mg·ml-1,1mg·ml-1 and 10mg·ml-1 three concentration of OD (405nm) values are less than positive control values, with the increased concentration of extracts, OD (405nm)-gradually decrease the value, indicating the number of TMV. This article in northwest of rhodiola crenulata ethanol extracts against TMV activity, Northwest of rhodiola crenulata demonstrated better TMV's infection and proliferation of TMV, the results, as in the rhodiola crenulata in targeted search out against TMV activity utility compounds and lay a good foundation.
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