Temporal And Spatial Expression Pattern Of Adipose Metabolism Related Genes In Lanzhou Fat-tailed Sheep

To elucidate temporal and spatial expression pattern of Lanzhou fat-tailed sheep (LZFTS) during growth and development, 7 candidate genes related to adipose metbolism were selected and their RNA transcripts were detected by quantitative real-time PCR (qPCR).30 lambs of LZFTS with 1 month-old were randomLy divided into six groups. Samples of greater omental fat, liver, muscle, hypodermic adipose, kidney peripheral adipose and tail adipose were collected after slaughtering animLs in the age of 1, 3, 5, 7, 9, 11 and 13 month-old, respectively. Total RNA were extracted using the improved method of TRIzol. cDNA was then synthesized by reverse transcription kit of SuperScriptTMⅢFirst -Strand Synthesis System for RT-PCR according to the manufacture's instruction.qPCR was used to analyse the stability of expression of the seven potential reference genes of 18S rRNA, GADPH, PGK1, SDHA, TUBB, YWHAZ and ACTB in various tissues and different developmental stages in LZFTS with GeNorm, Normfinder and BestKeeper. Expression of adipose metabolism related genes, FAS, DGAT1, HSL, FABP, UCP, G6PD and ACCαwere normalised using the geometric average of the two most stable housekeeping genes, either GADPH and TUBB2 or YWHAZ andTUBB2.The temporal and spatial expression pattern of FAS,DGAT1,HSL,FABP and G6PD in LZFTS have a quite different with the overal time mode of a rising RNA transcripts, then a drop, then another rise followed by another drop from the age of one month to thirtheen month-old. Expression of ACCα,HSL and G6PD are more significantly higher than that in other fat tissues. UPC3 is mainly expressed in liver and muscle, and is scarcely expressed in fat tissues. It was concluded that the genes of DGAT1, FAS, FABP, HSL and G6PD could be choices as candidate genes for further study of hypertrophic fat deposition in the tail of LZFTS.