Phylogenic Study Of Five Species Of Myxosporeans (Myxozoa: Myxosporea) Based On HSP70 Gene Data

Myxosporean parasites have a significant role as pathogens of freshwater and marine fishes in wild and cultured stocks with over 2,200 described species. Most species are identified on the basis of the myxospore. However, with the use of these classical zoological methods, it is very difficult to distinguish morphologically similar myxosporean species that may infect identical tissues and that develop in hosts species that are closely related taxonomically. Fortunately, molecular biological methods have become increasingly applied to parasitological studies and the taxonomic classification of myxosporeans has been expanded to incorporate phylogenetic analyses.In this experiment, we put investigations on 5 species: Zschokkella sp., Myxobolus ampullicapsulatus Zhao et al.,2008, Henneguya doneci Schulman, 1962 (3 population), Myxobolus branchicola sp.nov., Myxobolus hearti Chen, 1998.This work covered two main sections: one was the analysis of diversity of Henneguya doneci from the perspective of comparison of HSP70 gene. In this study, We determined the HSP70 gene sequences for 3 stocks of Henneguya doneci. The phylogeny trees were built By combining the HSP70 gene sequences of other 18 species available in the GenBank. Genetic differences among 3 stocks of Henneguya doneci and molecular phylogeny among some species were carefully compared. Through the obtained data, it's safe to say that HSP70 gene was a considerable mark with a strong conservative character.The other section was mainly focusing on the taxonomic and phylogenic relation of five myxosporean based on HSP70 gene. In the present study, the HSP70 gene sequences for 5 species were determined. By combining the HSP70 gene sequences of other 18 species available in the GenBank, we investigated the molecular phylogeny among some Myxosporidia species. The results of our study supported that HSP70 gene sequencing and identification of morphological characters which will enhance the taxonomy and systematics of Myxosporea.Information delivered from both of the two aspects of this study is of great value to reaserch on different myxosporean by advanced measurements.