Indentification And Screening Of Waterlogging Tolerance In Brassica Napus Mutated By EMS

Rapeseed is one of the most important oil crops in the world. However rapeseed has being faced with the problem of Waterlogging, so Waterlogging research about rapeseed is significant meaningful. Lots of comprehensive and deeply researches on Waterlogging tolerance ability have been conducted in rapeseed.The 1132 M3 generation rape seeds which were mutated by EMS including Zhongshuang 9 and GH18, were used in this experiment and their waterlogging tolerance were comprehensively estimated. Experiment results showed that EMS mutations caused wide variation. Selected by the seedling rate of the materials treated by waterlogging, there were 26 percent of mutations with the seedling rate higher than 50% and of these selected mutations, all the 5 humidity indexes were higher than the contrast, with coefficient of variation between 0.1066 and 0.4853. thus it was confirmed rich in variation. Graded for the waterlogging tolerance of these treated materials by their relative activity index,14 and 2 materials with strong high waterlogging tolerance were selected from the EMS mutations GH18 and 2 from Zhongshuang 9 respectively and these 16 Brassica materials would be used as excellent resources in waterlogging tolerance breeding.Rapes seedlings with waterlogging treatment, shoot fresh weight, soluble protein, root dry weight, shoot dry weight,5 characters waterlogging performance index were significantly decreased, while the leaf water holding capacity, root activity, membrane permeability, root dry weight, chlorophyll, soluble sugar, MDA and free proline 8 characters waterlogging performance index were significantly increased.67 seedlings tested in the humidity of the strongest material is L2120-1. Waterlogging tolerance abilities of L2120-1 were the strongest in 67 seedling material.In the bud stage and the screening periods 11 materials of L2120-1, L2135-2, L2125-1. L2091-2, L2018-1, L2110-1, L2807-3, L2540-3, L2112-2, L2127-3. L13108-1 were very good at waterlogging tolerance. The initial screening materials L2026-2, L2028-1, L2243-3, L2110-3, L2111-3 mutation were 5 false-positive material.