Reproductive Function Of Selenoprotein M In Chinese Mitten Crabs (Eriocheir Sinesis)

Chinese mitten crabs(Eriocheir sinensis) also known as hairy crab, fresh water crabs, they live mainly in the northern Asia,include western Korea and China.In China, this crab distributed from North of Yalu River in Liaoning to south in Xiamen. As early as 1000 years ago, Chinese people have got the living habits of Chinese mitten crabs, especially reproductive migratory habits.In recent years, the Chinese mitten crab as aspecialty of our country, nutrient-rich, unique flavor. Not only domestic well-known and renowned overseas, so our people to see it as aquatic treasures, and is widely farmed in China.Selenoproteins are present in all major forms of life, including eukaryotes, bacteria and archaea. In eukaryotic animals, selenoproteins often function as antioxidants, but rare or absent in other phyla, such as plants and fungi (except for the green alga Chlamydomonas). In recent years,the study of selenium has achieved significant results, a large number of literature Se and animals closely related to the reproductive system, Se in the absence of reproductive organs of male animals could lead to stunting, and affect sperm production, sperm number, sperm vitality, etc. For females, the lack of available research showed that Se can lead to disturbance of the female reproductive cycle of animals, fertility, reduced cub survival rate and lower.. Selenoprotein M (SelM) is a selenocysteine containing protein with redox activity, which is involved in the antioxidant response. However, information remains limited about SelM physiology and function in marine invertebrates, particularly in crustaceans. Hence, we investigated the reproductive functionality of SelM in the Chinese mitten crab(Eriocheir sinensis), which is a commercially important yet disease vulnerable aquaculture species. The full-length SelM cDNA (928 bp) strand was cloned by using PCR, based on an initial expressed sequence tag (EST) that was isolated from a hepatopancreatic cDNA library. The SelM cDNA contained a 390 bp open reading frame (ORF) that encoded a putative 129 amino acid (aa) protein. SelM mRNA expression in E. sinensis was (a) tissue-specific, with the highest expression observed in the hepatopancreas, testis, ovaries and intestines. Based on this information, we then detected the different stages of tissue expression for SelM in the testis, ovary, and male crab hepatopancreas and hemolymph. Overall, SelM was isolated successfully from the Chinese mitten crab, and its involvement in the regulation of reproduction during the period of rapid development in E.sinensis was confirmed.