| Rice can not be stored quite long as a grain variety. Cold and lack of oxygen is the condition for stored rice seed currently. This is uneconomical of consuming large amounts of energy and materials. From the realistic considering, it is necessary to increase the storability from the genetic angle of seed with biochemistry and molecular biology method. As a control material, Yunhui290 is the rice material of having much more storable. Compare the storage characteristics of common restored lines materials in production by using biochemical and proteomics methods. The results are shown as follows:(1) In this study, most commercial restore lines, including Hang1, Hang2, Minghui86, Minghui63, Duoxi1 and R527 were used as materials, and endurable storage material Yunhui290 as control. After water content equilibration, artificial aging in different stages were performed and the main components of these materials were measured by use of conventional chemical methods before and after aging treatment. The results of water content and germination rate showed that with the prolonging of aging period, the water content of materials increased gradually, in which Yunhui 290 exihibited the smallest and Hang 1 exhibited the largest.Meanwhile the germination rate decreased gradually , in which germination rate of Yunhui290 was 34%, whereas ot the materials completely lost their germ-ability agted 40d of artificial aging treatment with a germination rate of 0.(2) Balanced the seed moisture of the above materials, done with the artificial aging treatment of different period and determined the aging treatment and the material main chemical compositions with conventional chemical methods.The results showed that before and after aging treatment, each material exhibited no significant difference in the content of proteins and lipids, increased in that of starch and lipases. Proteomic analysis on four cultivars of different levels of germination ability, Yunhui290 and Hang1 respectively, is performed by using differential proteomics before and after aging treatment of 30d. The result obtained six proteins that may be related with the storage characteristics of rice.Two of them were LEA proteins. Meanwhile, this experiment will continue study them on the level of amino acid. CDNA sequence had obtained by amino acid sequence analysis of the relevant softwares. Had designed primer and extracted the rice seed RNA, gene cloning and function confirmation would be conducted.(3) Much observation was conducted in the process of seed treatment, and the results showed that some seeds had intact crusts with impairments inside, whereas others whose embryos were functional, looked impaired but with a good condition inside. On the part of the materials were kinds of shell paraffin section and scanning electron microscopy. The results indicated that the kind of shell is too hard to do paraffin section. Scanning electron microscopy results showed that shell upper and lower epidermis structures of Hang1 have changed after artificial aging treatment of 30d, stockade tissue of the exocuticula was loosed and the cutin membrane was much more transparent. Endepidermis was irregular and the arrangement didn't furl neatly after artificial aging treatment.(4) All of which indicates that the key factor to seed storage durability may not be the change of lipids in embryo but the impairments of the antioxidant defense system. The main reason impairing the antioxidant defense system in seed is the generation of H2O and hypoxia, which increases the content of water and the frequency of oxygen respiratory metabolism; therefore, metabolites and enzymes involved in the generation of H2O and hypoxia in seed metabolism are being measured to confirm those substances related to the increase of water content.
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