Development Of The Animal Model Of Hemagglutinating Encephalomyelitis Virus Infection By Respiratory Tract And Study On Its Dynamic Distribution

Porcine hemagglutinating encephalomyelitis is a kind of piglets disease caused by Hemagglutinating encephalomyelitis virus (HEV), showed neurological symptoms or vomiting and failure as the clinical symptoms of acute and highly contagious disease, mainly affects pigs from 1 to 3 weeks, the mortality rate was up to 20-100%. Serological survey showed that HEV infection was distributed by worldwide, and has caused great economic losses to the pig industry,but until now the studies on the pathogenicity of HEV was not deep enough, the dynamic distribution of the HEV in pigs was not clear,which impact the research of the HEV pathogenesis. So, we establish an animal model which can simulate the clinical symptoms and pathological changes, so that we can study the dynamic distribution and infection mechanisms in pigs.The establishment of this model is very important to HEV research and prevention.According to GenBank reported that HEV-67N strain of S gene sequences (AY078417)of conservative region designed a pair of specific primers, established quantitative fluorescence detection HEV RT-PCR, through the optimization of reaction conditions, using the established fluorescence quantitative RT-PCR to detect the virus content of each organization. The result shows that the virus mainly exited in the respiratory system and the nervous system, other organs also have the virus, but the levels was low, indicating that the virus is mainly transmitted through the respiratory system and have neurotropic nature.Using the Immunohistochemical technique to detect the distribution of the virus of the organizations, the results showed that within different infection time the distribution of virus in organs of the organizations was different, we also found that the positive signals in the virus of the brain were mainly located in the brain cortex of the pyramidal cells, the positive signals in the cerebellum virus located in the Purkinje cells,the positive signals in lung virus located in the alveolar epithelial cells, and we not found positive signals in other tissue samples.