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Nutritional Analysis Of The Rhizome Of Abelmoschus Sagittifolius (Kurz) Merr. And Effects Of Deflowerbud On Its Yield

Posted on:2012-09-17Degree:MasterType:Thesis
Country:ChinaCandidate:Z Q NanFull Text:PDF
GTID:2143330335956114Subject:Biochemistry and Molecular Biology
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Abelmoschus Sagittifolius(Kurz) Merr.), alias of HongHuaMaNing, also called the arrowleaf abelmoschus rhizome, Abelmoschus, one year or the perennial suffrutex shape herb, its rhizome is used as medicine. It can be eaten for a long time and won't cause posion and harmful effect. It may stimulates and awakes the brain, nourishes and builds up strength, and may also be used to treat neurasthenia, dizziness, the pain in waist and lower extremities, the stomach ache, the diarrhea and so on. In order to provide basic research results on cure elements and health care function, its nutritional component was detected and the effect on yield and antioxidase system was determined after flower bud removal. The results are as follows:1. Results showed that moisture content of arrowleaf abelmoschus rhizome was around 14.97%±0.37%, comparable with that of leguminous crops. Crude protein content was around 9.22%±0.09% which was slightly higher than that of maize and lower than that of common crops, soluble protein content was around 2.04%±0.05%. 17 sorts of and 15 sorts of amino acids were detected from hydrolysis samples and free samples, respectively, by L-8800 amino acid analyzer. Total contents of amino acids were 6.18% and 1.45% in hydrolysis samples and free samples, respectively, of which Asp content reached the highest amounts; essential amino acids and semi-essential amino acids accounted for 28.93% and 13.45% of total amino acid contents, respectively. Crude fat content of arrowleaf abelmoschus rhizome was 17.57%±0.11%, saturated fatty acids in arrowleaf abelmoschus rhizome accounted for 33.16% of total fatty acids, of which 27.20% was palmitic acids and 5.96% stearic acids. Unsaturated fatty acids accounted for 42.24% of total fatty acid contents, of which 8.46% was for oleic acids, 28.85% was for linoleic acids, 4.93% was for linolenic acids. Unknown fatty acids took up 24.59% of total fatty acid contents. Total sugar content of arrowleaf abelmoschus rhizome was 55.75%±0.80%, and soluble sugar content was 4.41%±0.23%, reducing sugar content was 2.08%±0.06%, the crude fiber content was 4.35%±0.19%; total sugar content was higher than that of soybean, while was lower than that of rice and flour and was equivalent to leguminous crops, such mung bean, pea etc.2. Measurements of spectrometry showed that contents of calcium, iron, zinc, manganese, copper, potassium, magnesium in arrowleaf abelmoschus rhizome were 399.60±25.55mg/100g,12.32±1.28mg/100g,5.35±0.07mg100g,1.40±0.06mg/100g, 1.15±0.05mg/100g,341.43±6.61mg/100g,129.78±1.10mg/100g,respectively. Mineral contents of arrowleaf abelmoschus rhizome were relatively higher compared with those of common crops, especially for calcium, iron, zinc contents.3. Study on impacts of flower bud removal on stem height and yield of arrowleaf abelmoschus rhizome showed that there existed no significant difference in heights, while yields of arrowleaf abelmoschus rhizome were significantly different. Yields of flower bud removal treatments were increased by 13.50% compared with CK.4. Results from isozyme electrophoresis experiments indicated that: 7 SOD bands were found in arrowleaf abelmoschus rhizome and flower bud removal treatments,but have slightly difference between them.5 POD bands were found in arrowleaf abelmoschus rhizome and bud removal treatments, from which at least 3 unknown bands were found.Measurements of isoenzymatic activity demonstrated that activities of SOD and POD under bud removal treatments were higher than those of CK treatments. There existed no visible CAT bands in enzyme electrophoresis experiments, there were little changes in CAT activity values when measurements proceeded, indicating CAT activity for these two treatments was both extremely weak.
Keywords/Search Tags:Abelmoschus sagittifolius (Kurz) Merr., rhizome, nutritional analysis, deflower-bud, yield, isozyme analysis
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