Comparative Gene Expression Profiles Of Leaves And Roots In Soybean Under Salt Stress And Normal Growth Conditions

With the rapid increasing of world population and the global area of salinized land has expanded each year, which seriously affect the cultivation of food crops production. The study on salt tolerance of soybean can increase soybean yield, thus, it can improve the salinization of land. In this study, after inbring line HJ-1 under NaCl stress (Hoagland solution with 120mM NaCl) for 24h till the leaves and roots of two-leaf stage seedlings of the soybean. Then using high-throughput Illumina sequencing technology to sequence the mRNA expression profile of soybean and analyze sequencing results with informatics. A large number of important key regulatory pathway genes on NaCl stress were obtained.10 genes selected from expression profiling data on the relative quantitative PCR verification analysis,And, these gene were significantly enriched GO functional gene cluster analysis and made significant enrichment Pathway statistical analysis of metabolic pathways.The main results are as follows:(i)A total of 874, up-regulated genes were differentially expressed in leaves and 1822, were up-regulated in roots under salt stress, respectively. Differentially co-expressed genes was 116 in roots and leaves. Most of the differentially co-expressed genes were involved in metabolism, transport, transcription and signal transduction,etc.A total of 578, up-regulated genes were regulated specifically in leaves and 1706 were specific to roots under salt stress, respectively.Through the analysis of specific differentially expressed genes, up-regulated genes in roots were significantly different, and in leaves due to downward trend were not obvious.(ii) Using software to classify these genes with GO functions were mainly involved in regulation of transcription factors, signal transduction genes, molecular chaperones, ion transmembrane proteins, oxidoreductases and proteins, enzymes and proteins of photosynthesis, Osmoregulation enzymes genes,etc.(iii) NaCl stress in some ABC transporter pathway and glutathione-related genes were upregulated.(iv) Taking TUB4 as a reference gene to apply quantitative PCR, relative expression data validated the differential expression of 10 genes, the results showed that NaCl stress and differential genes expression in control samples multiple of the basic sequencing were consistent with the expression profiling data, which verified expression profile is more credible.