The Establishment Of Perilla Frutescens Regeneration Stystem And Preliminary Research On Transformation System

Perilla cotyledon and hypocotyl were used as explants for tissue culture in this research. This study established vitro regeneration system for perilla and got normal regenerative plants. Comparative experimental methods were used to study the factors affecting the regeneration for perilla, while the perilla genetic transformation system were studied as a preliminary exploration,The conclusions were obtained as follows:1. Perilla cotyledons best differentiation medium was MS + BA 1.0mg/L + IAA 1.5 mg/L, the highest regeneration rate was 80.0%, every cotyledon germinated 2.3 buds. The perilla hypocotyl best differentiation medium was MS+BA 3.0mg/L + NAA 0.3 mg/L, the rate of regeneration was 70.9%, every cotyledon germinated 2.5 buds. Regeneration of shoots rooting medium was 1/2MS +1.0 mg/L BA +0.3 mg/L IAA, the rooting rate was up to 100% and the survival rate was more than 85%.2. 11-days aged perilla was used as experimental material, added sugar 30g/L. The perilla was transferred after precultured in 2,4-D, cotyledons precultured for 12 days in darkness and hypocotyl precultured for 8 days in darknessthen cultured at the condition of 14h/d light. Cultured in the medium added 4mg/L AgNO3, cotyledons explant regeneration rates improve 5.4% and hypocotyl explant regeneration rates improve 9.4%.3. Perilla hypocotyl selection pressure for regeneration was 40mg/L Kan, the rooting selective pressure was 35 mg/L Kan, The experment shows that every explant germinated 7.6 buds in the medium added Kan 15 mg/L. It is 3.3 times as control group, and greatly improve the number of regenerated shoots. After Agrobacterium infection, choose Carbenicillin as antibacterial agent, after two days co-culture, the explant transfered to the medium of 400 mg/L Car can get the best antimicrobial activity.