Differential Proteomic Analysis Of Lung Tissues Of Mice Infected By H5N1 Avian Influenza Virus

In 1997, highly pathogenic H5N1 avian influenza virus caused outbreaks in chickens in Hong Kong, and was transmitted to humans directly. H5N1 avian influenza viruses continue to evolve and spread and they are associated with widespread death in poultry, substantial economic loss to farmers, and reported infections of more than 500 people with a mortality rate of 60%. Therefore, to understand the genetic determinations of avian influenza virus pathogenicity and its ability to cross species barrier to mammalian hosts is of great importance.The process of disease is the result of pathogen-host interaction. Etiology studies have shown that multiple genes are involved in virulence of the highly pathogenic avian influenza virus (HPAIV), though to a particular virus a single gene may play a leading role. In order to further elucidate the molecular pathogenesis of the molecular mechanisms of HPAIV, we need to study the virus-host interaction during the infection. Protein is the executor of life activities. Therefore, to study the differences of protein expression profiles during the process of HPAIV infection could help us to further elucidate the molecular mechanisms of pathogenicity and provide ideas to find new drug target for influenza virus.Mice are important mammalian model for avian influenza virus, while the lung is a major target organ of influenza virus. In this study, the differentially expressed proteins was investigated in H5N1 avian influenza virus (HPAIV) infected mice by two dimensional gel electrophoresis (2-DE). The lung tissue lyses from the HPAIV infected or control groups were extracted at 72 hours post-inoculation and separated by 2-DE. Nine protein spots were found to have notably differential expression between the two groups(ratio>2,p<0.05). Seven of them were identified by MALDI-TOF/TOF, including ATP-dependent interferon response protein1 (ADIR1), interferon-induced protein with tetratricopeptide repeats 3(IFIT3), T cell receptor alpha TA27, Interferon-gamma-inducible p47 GTPase (IRG), Cytidine monophosphate kinase 2 (Cmpk2 protein), odorant receptor S86, myosin light chain 1/3. Moreover the transcriptional levels of 3 genes (ADIR, IFIT3 and IRG) were verified by real time PCR, which was completely consistent with the proteomic result. Our data could provide an insight into the AIV-host interactions.