Effects Of The Sublethal Doses Of Deltamethrin And Imidacloprid On Apis Mellifera Ligustica L. (Hymenoptera: Apidae)

As one of the most important economic insects, honeybees not only provide us bee honey and relational productions, but also, more significantly, pollinate nearly all of the crops for us. So honeybees play a vital role in the protection of crops, orchards, threatened plants, city garden, ecological restoration and so on. With the continuous improvement of insecticide and the emergence of new pesticides, the application of pesticides in area and intensity is being expanded, so honeybees will continuously be poisoned and some sublethal effects will be produced. In order to study the influence of insecticide on honeybees and carry out rational use of insecticides and provide basis for bees protection, sublethal effects of insecticide on honeybee was researched in the effects of sublethal doses of insecticide on the olfactory sensitivity of honeybee and the expression and distribution of the acetylcholine receptorα7 (nAChR-α7) in the brain of honeybee in the present study. The results are as follows.1,The sublethal doses of deltamethrin and imidacloprid to honeybees was defined;2,The effect regularity of sublethal doses of deltamethrin and imidacloprid on the olfactory sensitivity of the foraging bees was obtained. After oral consumption deltamethrin reduced sensitivity to antennal stimulation by sucrose solutions and water-induced proboscis extension reflex at doses of both 5ng/bee and 10ng/bee. Unlike deltamethrin, imidacloprid induced no effect in these olfactory sensitivity assays but increased water-induced proboscis extension reflex;3,The changing regularity of nAChR-α7 produced by sublethal doses of deltamethrin and imidacloprid in the brain of honeybees was defined. The distribution of nAChR-α7 in the brain of honeybee is very wide, especially in the mushrooms, optic lobes and olfactory lobes. Sublethal doses of imidacloprid induced down-regulation of nAChR-α7 in the brain, which is more obvious in the optic lobes by western blot;4,The relationship between sublethal doses of insecticide and the behaviour of honeybee was analysed combining the sublethal effects of insecticide on honeybees.