Genetics Analysis Of Near-isogenic Lines Resistant To Wheat Stripe Rust

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most destructive diseases of wheat worldwide. In China, where is the biggest epidemic zone in the world. It is the most destructive disease in autumn-sown wheat in northwestern and southwestern China. Destructive epidemics of stripe rust in China occurred in 1950,1964,1990,2002,2003 and 2009,which caused great yield losses. The release of rust resistant cultivars is considered to be the best option to control rust diseases. Alternate host of wheat stripe rust and the life history were identified, bring the research of wheat stripe rust into a new stage. However, utilizing wheat differential cultivars becomes the effective way of identifying the nosogenesis of race currently. Differential hosts consists of the single gene near-isogenic lines(NIL)to study the relationship between host and pathogen, can effectively avoid the complicated genetic background and interaction of multiple genes in traditional differential hosts. Not only will help to study the genetic composition and toxicity of pathogen population, but also will make international exchange. Providing useful information to breeding for disease resistance, and can improve the identification of Chinese race and the analysis of resistance to genic level.In this study, we reseached 9combinations from near-isogenic lines(NIL) resistant to wheat stripe rust which were established using the winter varieties Mingxian169 as a recurrent Parent,namely: Mingxian169/Chinese166,Mingxian169/T.spelta album,Mingxian169/Lee,Mingxian169/Compair,Mingxian169/Lovrin13,Mingxian169/Moro,Mingxian169/Kangyin655,Mingxian169/ Spaldings Prolific and Mingxian169/Resulka. In this paper used 3 methods to study the genes combinations and numbers of near-isogenic lines(NIL) resistant to wheat stripe rust, including resistant genepostulation, classic genetics and molecular marker analysis. The results as follows:Postulation experiments show that 8 combinations all contain the targeted resistant genes except for Mingxian169/Lee, and they are Mingxian169/Chinese,Mingxian169/T.spelta album,Mingxian169/Compair,Mingxian169/Lovrin13,Mingxian169/Moro,Mingxian169/Kangyin655,Mingxian169/Spaldings Prolific and Mingxian169/Resulka. On the base, according to the agrono-mic traits, such as plant height, reriod of growth and plant morphology, choose 20plants from 8combinations to be used as inheritance of resistance analysis experiment with1-2 Pst races. The results showed that the resistance of each plant of tasted combination todifferent Pst races was controlled by a pair of dominant genes, respectively. Mingxian169/Lee does not contain Yr7 resistant gene by molecular marker, another plants all contain single genenear-isogenic lines, tentatively entitled: Mingxian169*6/Yr1,Mingxian169*6/Yr5,Mingxian169*6/Yr8,Mingxian169*6/Yr9,Mingxian169*6/Yr10,Mingxian169*6/YrSpp,Mingxian 169*6/Yy2 and Mingxian169*6/YrRes.