Proteomics Studies Of Aspergilus Flavus Stress Response In Min Hua 6 Peanut Pod

Proteomics study is playing all increasing important role in the functional genomics era.Two-dimensional electrophoresis is one of the key technique in proteomics studies.Arachis hypogeae L. is susceptible to infection by Aspergillus flavus and pollute by aflatoxin. When the humidity, temperature and other environmental conditions are suitable, the peanuts are likely to be contaminated by aflatoxin in the process of pre-harvest, harvest and post-harvest storage, transportation and processing, which impacts on the improvement of the national economy greatly.when Aspergillus flavus infects peanut, the drought will be more conducive environment for the invasion of bacteria.It is great significant to explicit the resistance mechanisms of Aspergilus flavus infecting peanut pod, clarify the resistance gene function, achieve peanut high yield, disease-resistant molecular breeding. Many aspects were studied by scholars in the detection and prevention methods of Aspergillus infecting, the identification methods of peanut varieties resistance, electron microscopy ultrastructure, the physiological mechanisms of resistance, molecular marker, gene cloning and prokaryotic expression. There are not a lot of researchs about peanut pod, and, the research is very low about the mechanism of Aspergillus flavus infecting peanut pod on the base of gene and protein expression, especially about proteomics research. It will provide some basis for further understand the mechanism of Aspergillus flavus infecting peanut pod.Two-dimensional gel electrophoresis was employed to analyze the proteome from peanut pod of Min hua 6 after drought stress and inoculating Aspergilus flavus. Differentially expressed proteins were identified by MALDI/TOF/TOF-MS/MS and protein database searching. They are involved in pathogenesis-related protein, lection proteins and other functional proteins. This study will contribute to improve our understanding of the interaction mechanism of peanut pod and Aspergillus flavus, and lay the solid foundation for the further cloning and functional analysis of important genes peanut pod, as well as of anti-Aspergillus flavus new varieties of peanut. Specific results are as follows:1. Proteomic approaches were used to analyze the changes of differentially expressed proteins during different handles of peanut pod. Several low molecular weight proteins were detected by two-dimensional electrophoresis as being unique or significantly elevated. The identities of these proteins were established as pathogenesis-related proteins (pathogenesis-related proteins, PR 10). Our results suggest that these differentially phosphorylated PR 10 proteins may play an important role in mediating Aspergilus flavus infecting and drought stress responses. This information is conducive to study at the mechanism of peanut pod and drought factors and aflatoxin interaction in the basis of molecular level, and provide base for the study of mechanisms of peanut allergy and designing low-allergen vaccine.2. Two-dimensional gel electrophoresis were employed to analyze the plant lection protein from peanut pod of Min hua 6 after drought stress and inoculating Aspergilus flavus. Proteomic approaches were used to analyze the changes of differentially expressed proteins during different handles of peanut pod. There are 23 differential expression proteins. The identities of these proteins were established as Plant lection protein and precursor. In this study, lection proteins may be involved in the process of Aspergillus flavus infecting peanut pod, this research helps to understand the Aspergillus flavus lectin protein on the resistance mechanism.3. Two-dimensional gel electrophoresis was employed to analyze the proteome from peanut pod of Min hua 6 after drought stress and inoculating Aspergilus flavus. Proteomic approaches were used to analyze the changes of differentially expressed proteins during different handles of peanut pod. Forty-six differentially expressed proteins were detected by on 2-DE gels, and twenty-five were detected successfully. These proteins were involved in energy and metabolism, defense process, transformational process, secondary metabolic process, cell biological transformation and so on, which might relate to the process of drought stress and aflatoxin interaction. Overall, this study will contribute to improve our understanding of plant genetics and metabolism, and overall assist in the selection and breeding programs peanut improvement.