Fine Mapping Of Female Sterile Gene Fsv1 In Rice And Research Of The Embryo-sac Development

Fertility(or sterility) is a very important genetic and agronomic trait in rice. Identification the gene conferring fertility(or sterility) in rice and related theoretic study is important for geneticist and breeder.A high frequency female-sterile mutant (FSV1) of rice was found primarily from a mutant library created by ear-stem-injecting genomic DNA of Panicum maximum into a rice restorer line Gui99. Genetic analysis using a F2 population from a cross between FSV1 and a japonica line 02428 confirmed that the female-sterility was controlled by a pair of recessive gene (fsv1). With molecular markers, this study fine-mapped fsvl to a 100kb region on the long arm of chromosome 9, included 18 candidate genes.A study with the confocal laser scanning microscopy (CLSM) on the development of embryo-sac revealed that up to 85% megasporocyte aborted in FSV1.The paper also discussed the molecular mechanism of female-sterility formation and the application of FSV1.