The Study On In Vitro Antiviral Activity Of Cecropin A-bombesin Hybrid Peptide, Antibacterial Peptide Dermaseptin S4 And Calliphora Antiviral Peptide Alloferon1

Objective: The inhibition of Dermaseptin S4(DS4), CA-MA and Alloferon1 to Porcine reproductive and respiratory syndrome virus (PRRSV) and Newcastle disease virus(NDV) in vitro was studied, and the mechanism of action of which was probed initially. Finally, offering the theorial bases to manufacture the anti-medicine of PRRSV and NDV.Methods: Selecting the PRRSV and NDV which were proliferated by cell culture of Marc-145 and BHK-21 as the study target, using the different administration methods (such as meanwhile, before infection and after infection) and MTT to observe CPE, determine the D490nm and TCID50. According to the cell survival rate and the inhibition rate to PRRSV and NDV decided the inhibition degree of Dermaseptin S4(DS4), CA-MA and Alloferon1 to PRRSV and NDV.Results:First. There was inhibition of DS4 to PRRSV in vitro, and the inhibition degree was direct proporation whith their density(P<0.01). The inbition of DS4 to PRRSV was related with its type of action, and comparing among above three type of actions, the effect was the strongest of direct inactivation of DS4 to PRRSV.Second. There was inhibition of DS4 to NDV in vitro, and comparing among above three type of actions, the effect was the strongest of direct inactivation, but was not as good as the effect to PRRSV.Third. There was inhibition of CA-MA to PRRSV in vitro, but the effect was far not as good as the DS4, Alloferon1 and Lamivudine groups.Fourth. There was unvarying inhibition of CA-MA to NDV in vitro, and comparing among above three type of actions, the effect was the strongest of direct inactivation of CA-MA to NDV, but which was not as good as the effect of direct inactivation to PRRSV. But it was not obvious of absorption and interruption of NDV to target cells, and the inbition of proliferation of NDV in target cells.Fifth. There was inhibition of Alloferon1 to PRRSV in vitro, and the inhibition degree was direct proporation whith their density. comparing among above three type of actions, the effect was the strongest of direct inactivation of Alloferon1to PRRSV, but was not as good as DS4. But the absorption and interruption of Alloferon1to target cells of PRRSV, and he inbition of proliferation of PRRSVin target cells was stronger than the DS4 and CA-MA.Sixth. There was also inhibition of Alloferon1 to NDV in vitro, and the inhibition effect was obviously higher than the DS4 and CA-MA. comparing among above three type of actions, the effect was the strongest of direct inactivation of Alloferon1to NDV. Conclusion:First. There was unvarying inhibiton of DS4, Alloferon1 and CA-MA to PRRSV and NDV, and the nbition was related with their density.Second. The inbition of DS4, Alloferon1 and CA-MA to PRRSV and NDV was related with their type of actions, and the effect was the strongest of direct inactivation. Third. The effect was the strongest of direct inactivation of DS4 to PRRSV, and the inhibition of proliferation of NDV in target cells was the most weak.Fourth. The effect was the strongest of direct inactivation of Alloferon1 to NDV and the inhibition of proliferation of NDV in target cells was the most weak. But which was stronger than the DS4 and CA-MA.Fifth. The liveness was the strongest of rejection of Alloferon1 to PRRSV and NDV in vivro, and The liveness was the weakest of rejection of CA-MA to PRRSV and NDV in vivro.