Detection And Discrimination Of Honey And High Fructose Corn Syrup

Honey is a traditional health food which contains carbohydrates, proteins, amino acids, vitamins, trace elements in acid, pigment, bee pollen, hormones, active ingredients and so on. The various components and their content are quietly different due to various sources of honey, Even the same types of honey, many influencing factors such as Climatic conditions, soil composition, bee-keeping technology will cause the fails of analysis results which should be totally the same composition and the similar content. High Fructose Corn Syrup are hydrolysis from starch, the main composition and content is similar to the honey. So detection of complex of honey and High Fructose Corn Syrup isn't a easy work .But compared to High Fructose Corn Syrup come from starch which is hydrolysis by people , the main composition of honey and the ratio of those composition is consistent, the honey and High Fructose Corn Syrup will show some difference both in chemical and physical respects. Our aim is adopting Thin-layer chromatography(TLC) and Isozyme electrophoresis to identify Carbohydrate and Amylase in a convenient way. So we can tell difference between honey and High Fructose Corn Syrup. We take hand in The following aspects in honey detection.(1) Several conventional indicators, such as moisture, ash, and acidity are detected in our honey specimen. The content of aqua and reducing sugar take a combined effect on the viscosity of honey. Ash respect the content of protein in honey in some ways. Acidity is related to proportion monosaccharide. The mark of moisture, ash, and acidity are within the scope of the provisions, and we can perceive that the second syrup is more similar to the honey. But the result of those indicators show little substantive distinction between honey and High Fructose Corn Syrup, exist just as a normal indicator of quality determination.(2) We adopt Thin-layer chromatography(TLC) to distinguish carbohydrate of honey. above all we evaluate the content of glucose, sucrose, fructose and reducing sugar content in both honey and High Fructose Corn Syrup by Chemical titration. Then we got a quantitative assessment to the main composition of honey in both honey and High Fructose Corn Syrup. After that we analysis the carbohydrate by TLC, We compare ordinary silica gel G plates, high-performance silica gel G plate and hand-made plates, finally we select ordinary silica gel G plates as our stationary phase. In the selecting of mobile phase, We use three different systems as our show layer agents. Finally we adopt ethyl-acetate methanol-water-ammonia as the best show layer system. We regulate proportion of ethyl: acetate methanol: water: ammonia, and then we got the best ratio is 9:5:1:1.5. Under the condition, there is a conspicuity improvement on separation both in the honey and High Fructose Corn Syrup, and got a better effect compare to national standard. The spot showed on TLC increase from 3 to 6, and the effect of separation became better. The disparity of Rf between spot in the top and bottom increase from 0.18 to 0.31. we can easily perceive two extra spot on the TLC of High Fructose Corn Syrup .(3) Honey contain someα—Amylase and some self-converting enzyme, we test the activity of Amylase in honey. The way of ammonium persulfate extraction protein is adopted in our experiment. We appraise the consequent of protein extraction using distinctive saturation of ammonium persulfate refering to two aspect: Protein content and activity of Amylase, after that we got the best saturation of ammonium persulfate is forty percentage. Under that condition the protein content and activity of Amylase are the best. Finally we use the protein after extraction for Isozyme electrophoresis.