Scleroderma Polyrhizum Pers. Strains Of Pharmacognostic And Breeding, Fermentation Technology Research

Scleroderma polyrhizum Pers.is a kind of medicinal fungi. The records of Chinese and foreign literature has few people to compare their detailed and comprehensive study. In the end the tinctorius medicine as the Northeast, with more important clinical application. In this paper, such as the Submerged fermentation of traditional biotechnology methods, star crack hard Pima Bo on pharmacognosy, the separation of species, breeding, purification, and pharmacological effects have been studied for its medicinal value lay any application development the foundation.This paper using modern analytical testing technology to base source, the macroscopic and microscopic, to identify halloysitum rubrum (differential polysaccharide and protein), Use 5-bis nitro salicylic acid method to determine Scleroderma polyrhizum Pers. primary metabolites polysaccharide, thus determine fermentation time and put pot end time.Using tissue separating research strains of separation, purification, breeding, and all sorts of different conditions and medium inspection, For example: different source of carbon, nitrogen sources, trace elements, Different temperature, humidity and PH value, etc. In the single factor conditions determined after the orthogonal experiment, determined the best medium, Using liquid deep micro-organism fermentation mycelia and metabolites, namely the seeds, turn to the continued expansion of cultivation, investigation related conditions pick, obtains the best medium for 3% sucrose,2% specially designed,,0.1%KH₂SO₄ + MgSO₄; pH value for 5 8; temperature is 24℃; inoculation amount to 10%; speed for 160r/min, etc.Through the anti-inflammatory mouse auricular swelling experiment, and pain body torsion test, preliminary judgment, star crack crustaceous MaBoZi entities and fermented dry powder has anti-inflammatory pain after function, for better research whose pharmacology laid a good pharmacodynamic actions based.