Study On Spatio-temporal Distribution Of Arbuscular Mycorrhizal Fungi Of Hippophae Rhamnoides L.

Arbuscular mycorrhizal fungi which belong to the phylum Glomeromycota are ubiquitously associated with 80% of terrestrial plant species in natural ecosystems and the associations are of significant importance for the growth of plant partners. Especially, they can form a huge hyphae system, produce glomalin and have a positive impact on the formation and stabilization of soil aggregates.Hippophae rhamnoides L., belonging to Elaeapnacae Hippophae, has certain features such as well-developed root system, rapid growth. It is widely used for vegetation rehabilitation in arid areas for its high ecological and economic values.Soil samples were collected in Da Liangdi, Huang Liutiao and Duo Lun in farming–pastoral zone in the two northern provinces of China. To make full use of AM fungal resources and provide a new way to use Hippophae rhamnoides L., we did the research on the correlation between AM fungal and soil factors.1. The experiment separate 16 AMF species of 3 genera, 10 belong to Glomus, 5 belong to Acaulospora, 1 belongs to Scutellospora. The diversity of AM fungi is closely related with geographical environment, soil factors and host plants.2. The roots of Hippophae rhamnoides L. can be infected highly. The total colonizations of Da Liangdi, Huang Liutiao and Duo Lun are 88.7%,36.7% and 76.0%. The spore densities of the three sites are 973·100g^-1, 254·100g^-1 and 664·100g^-1. The result shows that AM fungi have a good symbiotic relation with Hippophae rhamnoides L..3. The total, hyphal, vesicular and arbuscular colonization of AM fungi have different trends. The highest total colonization and the hyphal colonization of three sampling sites appear in July. No obvious laws are found in vesicular colonization, arbuscular colonization and spore density. Distribution of AM fungi is influenced by geography and climate condition.4. The hyphal colonization, total colonization and spore density of Hippophae rhamnoides L. are different in different layers. The location of the site and the depth of soil have significant impact on AM fungal colonization rate. The highest total colonization, hyphal colonization and spore density appear in the layer of 0-30cm. The colonization of AM fungi is influenced by water content, oxygen content and soil nutrient.5. The distribution and colonization of AM fungi have a close relationship with soil factors. Spore density has a significant positive correlation with the total, hyphal and vesicular colonization. The total colonization, hyphal colonization, vesicular colonization and spore density have significant positive correlation with all the soil factors except acid phosphatase. Arbuscular colonization has a significant positive correlation with soil available N, urease, TEG and EEG. The distribution and colonization of AM fungi is related with soil factors closely.6. The total, hyphal, vesicular and arbuscular colonization of AM fungi have a significant correlation with microbial biomass C and N. The results prove that the soil factors can not only affect AM fungal colonization and spore production directly, but also affect AM fungi indirectly through affecting soil microbe.