Study On Biocontrol Of Cucumber Mould And Cucumber Anthracnose

Microorganisms from phyllosphere of cucumber were isolated and identified; The dual culture with 107 strains isolates were tested in vitro against Botrytis cinerea and Collectotrchum orbiculare . Pot experiment and field trial against B. cinerea and C.orbiculare were conducted.Pnminary study on biocontrol manchism against B. cinerea were done.1.The isolation and identification of microorganisms from phyllosphere of cucumber.A total of 25 and 4 strains fungi , 21 and 5 strains bacteria were isolated from the surface and interior of living leaves of cucumber. 25 and 4 strains fungi were identified as 13 and 3 fungal species, 21 and 5 strains bacteria as 10 and 4 bacterial species. 7 strains yeasts were obtained from the surface of living leaves of cucumber,which were identified 6 yeast species,and 35 strains actinomycetes were obtained, but only 1 strains were identified. lactic acid bacteria L2 was also identified.2.107strains of isolates were tested by dual culture in vitro against B. cinerea and C.orbiculare.We screened 9 strains of isolates with strong inhibiting effect.,including F22,with inhibitory rate 51.61% and 43.24% against B. cinerea and C. orbiculare; B5, with inhibitory rate 51.39% and 43.84%; S32,L2,S10,S24,St18,St61,S23, with inhibitory rate above 30% and above 40% against B. cinerea and C. orbicularï¼› S50, which have the best inhibitory effect only against C. orbicular, with inhibitory rate 64.79%.The inhibitory determination of fermented broth against B. cinerea showed B5 was the best, B5,L2,S32,S10 and S23 were superior to the solution of Procymidone at 1000g/L; against C. orbicular showed that S50 was the best, and that S50,L2, S24 diluted by 10 times , S32 dilutedby 5 times and B5,S23 and S10 with no dilution were superior or equal to 10000 units agricultural antibiotics 120.3.The result of pot experiment against B. cinerea indicated control effects of S32,L2,B5,S10 were better than others; against C. orbicular indicated control effects of S50,L2,B5,St61,S10,S32,St18 were better than others.The result of field trial against B. cinerea demonstrated control effects of B5,...