| Swine influenza is a kind of common respiratory infectious disease around the world. In early 1920s, it begins to spread in America, usually associating with other pathogenies, and resaulting in a serious harmfulness to hog industry. Since pig is so-called "the assembling containers" , Swine influenza has a important infection in public sanitation. Swine influenza virus (SIV) has many subtypes and they are mutable which brings many challenges for the diagnosis, prevention and cure. H1N1 is a conunon subtype of Swine influenza virus and it has been reported in some province of China. In order to find out its molecular changing regularity, develop an exact and sensitive diagnosic assay and offer in a base and reference for prevention of Swine influenza in molecular biology, we isolated some H1N1 substypes virus from hog farm in Henan province and established a real-time quantitative PCR diagnostic method which adapt to Swine influenza.The virus was named A/Swine/Henan/407/2005(H1N1), SHN407 for short, it is mutable when heated and sensitive to acids and can cause mouse sick even dead. Specific antibodies can be found in the blood of survived mouse, .The EID50, ELD50 are 10-6.5 and 10-1.75 respectively. Typical HA genes of H1N1 subtypes was downloaded around the world from Genbank and a pair of specific primers was designed in the relative conservative area outside encoding area. We amplyfied HA genes of SHN407 by RT-PCR, and cloned them into pGEM-T vector and then transfered them into E coli JM109. positive bacterium was sequenced after blue-white dot screening and identificating of plasmid PCR and evzyme digestion. Sequence analysis showed that the length of whole HA gene is 1701bp which encodes 566 amino acids, Including 330 a/nino acids in HAland 221 in HA2. there are 7 glucosylation sites totally, including 5 in HAland 2 in HA2. There is no basic amino acid around the cleavage site and no...
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