Construction Of An Expression Vector With Anti-disease Gene And Its Transformation In Wheat

Powdery mildew is a severe fungal disease in wheat, which greatly impacts the productivity and quality. Molecular machenism studies on plant disease resistance open a new pathway for plant disease resistance improvement. Disease resistance genes, pathogenesis related protein genes and key signal genes in plant disease resistance reaction may be aim sites to be engineering regulated for plant disease resistance improvement. Wheat (Triticum aestivum L.) TaLRK gene encodes a receptor-like kinase, TaEDR1 encodes a MAP kinase kinase kinase (MAPKKK), both may be key members in signal pathway of wheat disease resistance reaction. This study was to regulate the expressions of TaLRK and TaEDR1 by biological engineering for improving wheat powdery mildew resistance. The results are listing as following:1. Plant expression vector construction: pAHC25 was used as a basic vector, the plant over-expression vectors of pAH-TaLRK⁽⁺⁾, pAH-5- TER and pAH- R- TER were constructed.2. Obtained transgenic plants: The expression vectors were transferred into powdery mildew susceptible wheat cultivar"zhoumai18"by pollen-tuber pathway. Transgenic plants of T₀ generation were obtained by PPT-resistance screening and PCR detection.3. Metabolic characterization and powdery mildew resistance ability of the transgenic plants: The disease resistance ability and the protect enzyme system of the transgenic plants, control plants and disease resistant varieties of wheat were compared after Blumeria graminis (DC.) E.O. Speer f. sp. tritici infection. The results showed that parts transgenic plants were increased 2-3 in powdery mildew resistance.