Study On The Superovulation And Embryo Transfer Of Mouse

Objective: In order to apply superovulation and embryo transfer for controlling the mouse's offspring and therefor use these techniques on other animals, to expedite the species or breed improvement and increase the usage rate of choiceness variety, the superovulation and embryo transfer techniques of mouse were manipulated.Methods: Adult mice of Kunming variety were used as experiment animals. After judgement of oestrus, the oestrus period and oestrus stage were determined, and then those mouse were grouped depending on oestrus stage. The mice were dealed with diferrent dosage of PMSG and HCG to make them superovulation, afterwords were mated individually with the male mouse together at same cage. The embryoes were rinsed out and evaluated after 3.5 days when the plug had been seen. The transplant mice were born after the high-quality embryoes were transferred into the receptor's mouse uterus at the same oestrus stage which was stimulated by mating with the male mouse which spermaduct had been ligated previously.Results: The oestrus cycle of 157 mice were investigated. One hundred and eight mice were superovulated according to their different stage of oestrus cycle, the result was that number and quality of embryos superovulated in earlier oestrus stage excelled those in other stage. Quality embryos superovulated were chosen and implanted into receptor's uterus, which succeeded twice,9 transplant offsprings were bron.Conclusion: The better results may obtained when the mice were superovulated at the early oestrus stage and the later stage of the oestrus cycle, however the best was at the early oestrus stage. The embryoes should be implanted into both sides of the uterus. The whole transplant is very important for success.