Mass Production Technology For Entomopathogenic Nematodes

Entomopathogenic nematodes are very promising biological control factor since the early of 20 century. Application of entomopathogenic nematodes was limited because of faulty mass production. Several producing methods were adopted to meet different market's need in this test. In order to find logical technology, yield, quality, cost and efficiency of entomopathogenic nematodes were estimated. The results provided academic evidence and practical direction.This paper was completed at Northeast Institute of Geography and Agricultural Ecology Chinese Academic Science. Traditional White trap method was using Galleria mellonella as host insect, at the same time, Heterorhabditis bacteriophora-1 (Hb-1) and Steinernema carpocapsae-2 (Sc-2) were cultivated by Lowtek, liquid and solid production methods, respectively. The results were as follows:Tenebrio molitor was used as host on Lowtek production method. The results showed that vermiculite was better substrate, that doses of Hb-1 and Sc-2 were 800 and 1600 infective juveniles pre gram larva, respectively. The proportion of substrate and the insect was 3:2. The sterile substrate and turnover bags were unnecessary. The Hb-1 was collected at 12d and 15d, but Sc-2 at 10d and 13d. The storage period of time of filtered nematode was longer than that of direct storage.The yield and efficiency of inoculated mono-nematodes were more higher than surface sterile ones during TSYS liquid, and more higher TSYS than PSYG Hb-1 produced by TSYS liquid and solid production was collected at the nematodes inoculated 17d. Sc-2 was collected at 12d by TSYS liquid culture, but lid by solid culture.The yield and quality of nematodes by different producing methods were examined, the results showed that the yield of solid production was the best, then liquid production. The yield of White trap and Lowtek was lower, and wasn't significantly. The quality of the nematode produced by four methods was good. Corrected mortality of Galleria mellonella was higher significantly by the nematodes produced by White trap than other methods at 12h, but solid production and Lowtek was almost same at 48h. The cost of nematodes produced by Lowtek and solid production methods was lower than liquid and White trap methods, and the efficiency of solid production was the highest. The sequence of the yield and efficiency of Hb-1 and Sc-2 was Solid production > Liquid production > White trap > Lowtek. The sequence of quality was White trap > Lowtek > Solid production > Liquid production. The sequence of cost was Lowtek < Solid production < White trap < Liquid production...