Isolation And Characterization Of Yak Endometrial Glandular Cell And Stomal Cell In Vitro

To establish a culture system and get the specific markers for Yak Endometrial Glandular cell and Stromal cell,the distribution of Cytokeratins , CK7 , vimentin and Actin in the normal uterus and the gravid uterus of Yak were investigated immunohistochemically. In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus. In the gravid uterus, The CKs immunolabelling were detected in glandular cell, luminal epithelial cell, traphoblast cell, endoblastic cell and allantoic cell; Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus but in endoblastic cell and some luminal epithelial cell. It is an effective way to separate the stromal cell from the glandular cell that the tissue was digested by 0.1% collagenase, and then tissue digest was passed over a stacked sterile wire sieve assembly with number 100 wire sieve, followed by a number 400 wire sieve. The viability of stromal cell was 90%, The viability of glandular cell was 85%. YSC and YGE was cultured and propagated in RPMI1640 for at least 7 and 10 population doublings. There is at least 20% and 30% FBS in culture media for YSC and YGE growing normally. The CKs was detected in YGE, and the Vimentin was dected in YSC; and the charactorzation was reminded by propagating, in vitro. There were not CK7 in YGE and YSC.