The Establishment Of An Silkgland Cell Line BmSG-SWU1 Of Silkworm And Study On RAPD And ISSR Thereof

Objective: After having drawn the frame of silkworm genome, functional genome researches are in great need of providing numerous cell lines of silkworm as research platform. In order to meet with these requirements in cytobiology, developmental biology and molecular biology, establishment of new baculovirus expression vector systems and study on cell engineering in silkworm and transgenic silkworm, we conducted intensive researches on establishing and studying new cell lines of slikworm.The silk gland is one of silkworm most important organs, Usually, the silk gland is thought to be one kind of ideal model system which product sole protein, Therefore, this kind of cell raise in vitro will be helpful to research biosynthesis mechanism of sericin and the sericin. Through Bombyx mori .silk gland cell raise, we can attempt to develop one kind of production system of sericin in vitro, the silk gland cells stop differentiation in the time of larva, therefore the silk gland cells of larva are terminal differentiation cellsMethod: This experiment material is silkworm Xia Fang×Qiu Bai. Take newly-hatched silkworm silk gland which just was born, after processing vaccinates to the cell bottle. The gain Bombyx mori. silk gland cell, by the Grace nutrient fluid in vitro culture, then after 150d for primary culture, passage was taken, later each approximately 15d take a passage, after that, the time of passage cut to 10 day. When the time of passage stabilized in 7 day, stable cell according to cell line establishment standard examination cell line a series of biologic characteristic.Result: on to 25 generations.The cell assumes the expotential growth in inoculating for 168h, the critical multiplication density is 1.0×10~6 cells/Milliliter. The chromosomes of BmSG-SWU1 cell line was typical of Lepidoptera insects, namely the chromosomes in metaphase were short pole-like or granule-like and the centromeres were dispersed-type. The ratio of duple cells was 76.28%, thus, it belongs to duple ones. It suggested that the cell line well kept the characteristics of the mitosis and the...