The Effects Of Immunepreparations, Antibiotic And Probiotics In Nonspecific Immune System In Litopenaeus Vannamei

The study includes two parts : 1 The effect of three immunepreparations as feed additives on nonspecific immune system of pacific white shrimp, Litopenaeus vannamei 2 The effect of antibiotic and probiotics on nonspecific immune system of pacific white shrimp, Litopenaeus vannamei and the influence of antibiotic and effective on growth of L. vannamei and farming environment.Part One : Litopenaeus vannamei, 6-8cm in body length were divided into four groups randomly, and feed basic diet (control group) , basic diet + 5%MB, basic diet + 0.2% immune polysaccharide, and basic diet + 0.1% yuxiakangbao for 2 months respectively, each treatment was run in triplicate, Sampling was half a month. The bacteriolytic activities(U_L), antibacterial activities(U_a), phenoloxidase activities(PO) and alkaline phosphatase activities(ALP) in the serum were conducted to determine the effects of the humoral immune factors on L.vannamei fed with the three immune preparations. The results showed the activities of U_L, U_a, PO and ALP in the serum of L.vannamei of the experimental groups were higher than those of the control group generally, indicating the effectiveness of the three immune preparations in enhancing the nonspecific immune system of L.vannamei.Part Two : The experiment began on June 30,2005 and included antibiotic group which were added 2mg·L^-1 chloramphenicol to farming water, probiotics group which were added 90g lishenghuojun and 18ml EM to farming water and control group which were added nothing to farming water. Each treatment was run in duplicate and the experiment last for a month and detecting contents included water quality factors(water temperature, pH,DO and so on ) , phytoplankton, vibrio amounts and bacteria amounts in farming water, substrate and shrimp intestine, immune factors in shrimp serum, muscle and hepatapancreas and so on. The results showed : 1 chloramphenicol destroyed the farming environment and made pH, DO and nutrients change violently and increased COD in the farming water and had a disadvantage on growth of L.vannamei, but probiotics could stabilize pH, and could enhance the growth of L.vannamei, but probiotics could not play an obviously role on decreasing...