Studies On The Taxol-producing Of Taxus Endophytic Fungus

This study focused on taxus and their endophytic Fungi. The qualitative and quantitative characters of the Taxol-producing endophytic fungi had been analyzed. The evolutionary and genetic bases also had been discussed while we amplified for the first time a fragment of the gene coding BAPT (C-13phenylpropanoid side chain-CoA acyltransferase), one of the key enzymes in the taxol biosynthesis pathway from the fungi .At the same time, we established an easy and economical method to screen taxol-producing endophytic fungus. Introducing a VHB gene into one of the strains observably improved the growth rate in an oxygen limited condition. It provided good values to produce taxol by using zymotechnics.1 Isolation and purification of taxus endophytic fungiWe put the yew branches into triangle bottle with MS culture medium after surface sterilizer. Since the endophytic fungi growth up, its hypha tip had been picked up and put into a plat with CYM culture medium. The purification substance also gained by this way after more than 5 times to be repeated .2 Identification of the Taxol-producing endophytic FungiThe qualitative and quantitative characters of the Taxol-producing endophytic fungi had been analyzed to three fungi which had been identified that having the capability to produce taxol via the traditional method TLC and HPLC(8.5,31.5,31.1μg/L).Based on the 18SrDNA conservative sequence, we designed a pair of primes and gained the right sequence-target. The result and the fungi facets reveal that they should be attributed to two genus, Fusarium and Pestalotiopsis.3 Identification and pilot study of taxus engophytic fungi's taxol-producing correlation gene BAPTBased on the BAPT conservative sequence, we designed a pair of prime and gained the right sequence-target. Sequence and BLAST analysis provided that the evolutionary and genetic bases should gene transfer rather than coevolution.At the same time, we established an easy and economical method to screen taxol-producing endophytic fungus.4 Gene mend of the fungus by electric transferThe plasmid PBG1V included the VHB gene had been transferd into one of the...