Reseach About Amplification And Expressing Vector Construction Of Plant Broad-spectrum Disease-resistance Gene

The horticulture plants usually are cultivated in the greenroom where are easy to cause many bacterial and fungal diseases. Once were infected by the all kinds of diseases, the horticulture plants'value of view and admire was seriously affected and the economy value was seriously reduced.Systematic acquired resistance (SAR) is the most effective way in plant resistance to pathogens. Using the key gene in systematic acquired resistance (SAR) signal transduction, we can introduced the plants own disease-resistance which can gain a broad-spectrum disease resistance. NPR1 genes play an important role in the plant SAR. Over-expressing NPR1 gene show high resistance to many pathogens and the transgenic plants with over-express NPR1 gene also enhanced its resistance to a broad-spectrum disease. They found: In the downstream of signals transmission channels, there are a lot of same transcription factors which join in the defensive reaction induced by differently resistant gene. The TGA2 transcription factor can directly combine with most of pathogens-related gene and enhance the express level of pathogens-related protein. TGA2 transcription factor synergized with NPR1 gene can induce the plants SAR which can specially induce the pathogenesis-related protein and achieved the efforts of a broad-spectrum disease resistance.The main results were summarized as follows:1. NPR1 gene was cloned from Arabidopsis thaliana by RT-PCR. The result of sequence analysis showed that the length of gene was 1782bp .Compared with the published sequence in the NCBI they are 100% homologous.2. TGA2 transcription factor was cloned from Arabidopsis thaliana by RT-PCR. The result of sequence analysis showed that the length of transcription factor was 993bp .Compared with the published sequence in the NCBI they are 99.8% homologous. There were two nucleic acids permutations, but permutations were out of the function area of transcription factor.3. In the experiment, we insert the NPR1gene into the position which is between CaMV35S promoter and T-nos terminator of media expression vectors. We achieved the p35ST-TGA2 plant expression vectors4. In the experiment, we insert the TGA2 transcription factor into the position which is between CaMV35S promoter and T-nos terminator of media expression vectors. We achieved the...