Cloning And Morlecular Character Of Phosphate Deficiency Specially Induced Genes TaIPS In Wheat And Comparison In Two Varaties With Different Phosphate Efficiency

IPSs (induced by Pi starvation)are non-protein coding genes, which respond rapidly and specifically to phosphorous (P) deficiency. Analysis of IPS genes of wheat (Triticum aestivum L.) will enhance the understanding of the genetic response to P starvation and the developing of molecular diagnosis of P deficiency in wheat. In this study, through comparative genomics strategy between wheat and barley(Hordeum vulgare), three TaIPS1 genes (TaIPS1.1, TaIPS1.2 and TaIPS1.3) and two TaIPS2 genes (TaIPS2.1 and TaIPS2.2) were cloned in wheat. The genes specific character on P deficiency, and interaction of N with P were specially studed using RT-PCR. The signal regulation of TaIPS1 genes were analysized also by divided-roots experiment. Besids, phosphate efficiency and the expression of TaIPS1s were compared in two wheats, and the genes were finally physically located on the chromosomes of wheat"Chinese Spring". The results are as follows:P deficiency but not any other elements, is strongly increased the expressions of TaIPS1 genes in roots, while it is moderately increased the expressions of two TaIPS2 genes in roots and TaIPS1.1 in shoots, and is slightly increased the expressions of TaIPS1.2 and the two TaIPS2 genes in shoots. N deficiency completely repressed the responses of the three TaIPS1 genes to P deficiency in both roots and shoots, but had no significant influence on the expressions of the two TaIPS2 genes, suggesting that responses of some TaIPS genes to P deficiency depends on N status in wheat plants. It is interesting that TaIPS1.3 was induced by P deficiency in roots, but unexpectedly became compositive expression in lesves or shoot. Divided root experiment showed that TaIPS1 were mainly regulated by local signal instead of systemic signal of plant. The lower local Pi concentration is, the stronger expression of TaIPS1 is."Xiaoyan54"is a high-afficient phosphate genotype wheat, while"Jing411"is a low-afficient phosphate genotype wheat."Xiaoyan54"displayed higher phosphate efficiency...